{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T07:33:37Z","timestamp":1781249617167,"version":"3.54.1"},"reference-count":14,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. Gelatin-thrombin matrix (GTM) tissue sealant use was previously identified as an independent predictor of pelvic infection following hysterectomies. We aim to elucidate contributing factors by assessing influence of GTM on bacterial colony formation and characterizing bacteria present at the vaginal cuff.<jats:italic>Methods<\/jats:italic>.<jats:italic>Escherichia coli<\/jats:italic>was incubated in phosphate-buffered saline (PBS) and pelvic washings with and without GTM to assess influence on colony formation. Pelvic washings of the vaginal cuff were collected from hysterectomies occurring from June through October 2015.<jats:italic>In vitro<\/jats:italic>techniques, 16S rRNA gene qPCR, and 16S amplicon sequencing were performed with washings to characterize bacteria at the vaginal cuff.<jats:italic>Results<\/jats:italic>. Mean bacterial colony formation in PBS was greater for<jats:italic>E. coli<\/jats:italic>incubated in the presence of GTM (1.48 \u00d7 10<jats:sup>7<\/jats:sup>\u2009CFU\/mL) versus without (9.95 \u00d7 10<jats:sup>5<\/jats:sup>\u2009CFU\/mL) following 20-hour incubation (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>). Out of 61 pelvic washings samples, 3 were culture positive (\u22655000\u2009CFU\/mL) with<jats:italic>Enterococcus faecalis<\/jats:italic>.<jats:italic>Conclusion<\/jats:italic>.<jats:italic>In vitro<\/jats:italic>experiments support a facilitating role of GTM on colony formation of<jats:italic>E. coli<\/jats:italic>in PBS. However, given the negative results of surgical site washings following adequate disinfection, the role of GTM in promoting posthysterectomy pelvic infections may be limited. Analysis of pelvic washings revealed presence of<jats:italic>E. faecalis<\/jats:italic>, but results were inconclusive. Further studies are recommended.<\/jats:p>","DOI":"10.1155\/2016\/2649708","type":"journal-article","created":{"date-parts":[[2016,4,21]],"date-time":"2016-04-21T17:00:42Z","timestamp":1461258042000},"page":"1-6","source":"Crossref","is-referenced-by-count":1,"title":["Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection"],"prefix":"10.1155","volume":"2016","author":[{"given":"Michael J.","family":"Jarrett","sequence":"first","affiliation":[{"name":"Research Track, University of Colorado School of Medicine, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"V\u00e1zquez-Torres","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel N.","family":"Frank","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce D.","family":"McCollister","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick K.","family":"Henthorn","sequence":"additional","affiliation":[{"name":"Research Track, University of Colorado School of Medicine, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Ir","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kian","family":"Behbakht","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology, Department of OB\/GYN, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, 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