{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:23:50Z","timestamp":1740173030413,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Charles University research project","award":["P25\/LF1\/2"],"award-info":[{"award-number":["P25\/LF1\/2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Objective.<\/jats:italic> To determine the expression of chemokine receptors in lymphocytes from thyroid nodules and peripheral blood in patients with and without Hashimoto\u2019s thyroiditis (HT).<jats:italic> Patients and Methods.<\/jats:italic> The study included 46 women with thyroid nodules and HT and 60 women with thyroid nodules without HT (controls) who underwent a fine needle aspiration biopsy (FNAB). Expression of chemokine receptors CXCR3, CCR5, and CRTH2 was assessed by flow cytometry in lymphocytes from FNAB samples and from peripheral blood.<jats:italic> Results.<\/jats:italic> The percentage of CRTH2+ lymphocytes was higher in nodules with HT in comparison with controls, both in FNAB samples (13.95 versus 6.7%, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.008<\/mml:mn><\/mml:math>) and in peripheral blood (6.7 versus 5.13%, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.047<\/mml:mn><\/mml:math>), and positively correlated with serum antibodies to thyroid peroxidase (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>r<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.243<\/mml:mn><\/mml:math>; <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.026<\/mml:mn><\/mml:math>) and negatively correlated with thyroid volume (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>r<\/mml:mi><mml:mo>=<\/mml:mo><mml:mo>-<\/mml:mo><mml:mn fontstyle=\"italic\">0.346<\/mml:mn><\/mml:math>; <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.008<\/mml:mn><\/mml:math>). Lymphocytes from neoplastic nodules showed a higher expression of both CXCR3 and CCR5 than those from hyperplastic ones.<jats:italic> Conclusion.<\/jats:italic> Flow cytometry performed in FNAB samples may serve as a good tool in investigation of intrathyroidal expression of immunological parameters. In our study, the CRTH2 expression on thyroid-infiltrating lymphocytes as well as on lymphocytes from peripheral blood was increased in HT as compared to controls.<\/jats:p>","DOI":"10.1155\/2016\/2743614","type":"journal-article","created":{"date-parts":[[2016,10,31]],"date-time":"2016-10-31T17:00:49Z","timestamp":1477933249000},"page":"1-7","source":"Crossref","is-referenced-by-count":3,"title":["CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto\u2019s Thyroiditis Obtained by Fine Needle Aspiration Biopsy"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0920-4854","authenticated-orcid":true,"given":"Jan","family":"Jiskra","sequence":"first","affiliation":[{"name":"Third Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech 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Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zde\u0148ka","family":"L\u00edmanov\u00e1","sequence":"additional","affiliation":[{"name":"Third Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Mare\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Medical Biochemistry and Laboratory Diagnostics, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eli\u0161ka","family":"Potlukov\u00e1","sequence":"additional","affiliation":[{"name":"Third Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech Republic"},{"name":"Division of Internal 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