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Hypoxia\u2010induced sustained contraction of porcine coronary artery is endothelium\u2010independent and mediated by PI3K\/Akt\/Rho kinase. Nitroglycerin (NTG) is a vasodilator used to treat angina pectoris and acute heart failure. The present study was to determine the role of NTG in hypoxia\u2010induced endothelium\u2010independent contraction and the underlying mechanism.<jats:italic>Methods and Results<\/jats:italic>. Organ chamber technique was used to measure the isometric vessel tension of isolated porcine coronary arteries. Protein levels of phosphorylated and total Akt were determined by western blot. A sustained contraction of porcine coronary arteries induced by hypoxia was significantly reduced by NTG but not by isoproterenol. This contraction was also inhibited by DETA NONOate, 8\u2010Br\u2010cGMP, which can be reversed by ODQ, and Rp\u20108\u2010Br\u2010PET\u2010cGMPS. The restored contraction was blocked by LY294002. The reduction of Akt\u2010p at Ser\u2010473 by NTG, DETA NONOate, and 8\u2010Br\u2010cGMP was significantly inhibited by ODQ, PKG\u2010I. The decrease in Akt\u2010p level by NTG and 8\u2010Br\u2010cGMP was prevented by calyculin A but not by okadaic acid.<jats:italic>Conclusions<\/jats:italic>. These results demonstrated that the endothelium\u2010independent sustained hypoxic vasoconstriction can be prevented by NTG and that the inhibition of PI3K\/Akt signaling pathway may be involved.<\/jats:p>","DOI":"10.1155\/2016\/2916017","type":"journal-article","created":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T21:02:33Z","timestamp":1451422953000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Endothelium\u2010Independent Hypoxic Contraction Is Prevented Specifically by Nitroglycerin via Inhibition of Akt Kinase in Porcine Coronary Artery"],"prefix":"10.1155","volume":"2016","author":[{"given":"Huixia","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanming","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peixin","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liling","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuansheng","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dou","family":"Dou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,29]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1976.230.5.1261"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(84)90377-6"},{"key":"e_1_2_8_3_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0022-3565(25)32040-9","article-title":"Inhibitory effect of lidoflazine on contractions of isolated canine coronary arteries caused by norepinephrine, 5-hydroxytryptamine, high potassium, anoxia and ergonovine maleate","volume":"213","author":"Van Nuete J. 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