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Herein, we determined the role of the neutrophil during the early stages of experimental pulmonary paracoccidioidomycosis using a monoclonal antibody (mAb) specific for neutrophils. Male BALB\/c mice were inoculated intranasally with<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn fontstyle=\"italic\">1.5<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn fontstyle=\"italic\">10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mn mathvariant=\"normal\">6<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>or<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn fontstyle=\"italic\">2<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn fontstyle=\"italic\">10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mn mathvariant=\"normal\">6<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math><jats:italic>P. brasiliensis<\/jats:italic>yeast cells. The mAb was administered 24\u2009h before infection, followed by doses every 48\u2009h until mice were sacrificed. Survival time was evaluated and mice were sacrificed at 48\u2009h and 96\u2009h after inoculation to assess cellularity, fungal load, cytokine\/chemokine levels, and histopathological analysis. Neutrophils from mAb-treated mice were efficiently depleted (99.04%). Eighty percent of the mice treated with the mAb and infected with<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mn fontstyle=\"italic\">1.5<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn fontstyle=\"italic\">10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mn mathvariant=\"normal\">6<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>yeast cells died during the first two weeks after infection. When mice were treated and infected with<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mn fontstyle=\"italic\">2<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn fontstyle=\"italic\">10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mn mathvariant=\"normal\">6<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>yeast cells, 100% of them succumbed by the first week after infection. During the acute inflammatory response significant increases in numbers of eosinophils, fungal load and levels of proinflammatory cytokines\/chemokines were observed in the mAb-treated mice. We also confirmed that neutrophils are an important source of IFN-<jats:italic>\u03b3<\/jats:italic>and IL-17. These results indicate that neutrophils are essential for protection as well as being important for regulating the early inflammatory immune response in experimental pulmonary paracoccidioidomycosis.<\/jats:p>","DOI":"10.1155\/2016\/3183285","type":"journal-article","created":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T21:02:41Z","timestamp":1472158961000},"page":"1-17","source":"Crossref","is-referenced-by-count":12,"title":["Depletion of Neutrophils Exacerbates the Early Inflammatory Immune Response in Lungs of Mice Infected with<i>Paracoccidioides brasiliensis<\/i>"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7983-1293","authenticated-orcid":true,"given":"Paula Andrea","family":"Pino-Tamayo","sequence":"first","affiliation":[{"name":"Medical and Experimental Mycology Unit, Corporaci\u00f3n para Investigaciones Biol\u00f3gicas (CIB), Medell\u00edn, 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