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We report that peripheral blood mononuclear cells (PBMCs), derived from a female LHON patient with bilateral reduced vision and carrying the pathogenic mutation 11778\/ND4, display increased levels of reactive oxygen species  (ROS) and reactive  nitrogen species (RNS), as revealed by flow cytometry, fluorometric measurements of nitrite\/nitrate, and 3\u2010nitrotyrosine immunodetection. Moreover, viability assays with the tetrazolium dye MTT showed that lymphoblasts from the same patient are more sensitive to prolonged NO exposure, leading to cell death. Taken together these findings suggest that oxidative and nitrosative stress cooperatively play an important role in driving LHON pathology when excess NO remains available over time in the cell environment.<\/jats:p>","DOI":"10.1155\/2016\/3187560","type":"journal-article","created":{"date-parts":[[2015,12,31]],"date-time":"2015-12-31T21:04:22Z","timestamp":1451595862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Evidence for Detrimental Cross Interactions between Reactive Oxygen and Nitrogen Species in Leber\u2019s Hereditary Optic Neuropathy 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