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Resistance of <jats:italic>C. albicans<\/jats:italic> to antifungal agents and limited antifungal agents has potentially serious implications for management of infections. As a famous multiherb prescription in China, Huanglian Jiedu Decoction (HLJJD, <jats:italic>Orengedokuto<\/jats:italic> in Japan) is efficient against <jats:italic>Trichophyton mentagrophytes<\/jats:italic> and <jats:italic>C. albicans<\/jats:italic>. But the antifungal mechanism of HLJDD remains unclear. In this study, by using RNA\u2010seq technique, we performed a transcriptomics analysis of gene expression changes for <jats:italic>C. albicans<\/jats:italic> under the treatment of HLJDD. A total of 6057 predicted protein\u2010encoding genes were identified. By gene expression analysis, we obtained a total of 735 differentially expressed genes (DEGs), including 700 upregulated genes and 35 downregulated genes. Genes encoding multidrug transporters such as ABC transporter and MFS transporter were identified to be significantly upregulated. Meanwhile, by pathway enrichment analysis, we identified 26 significant pathways, in which pathways of DNA replication and transporter activity were mainly involved. These results might provide insights for the inhibition mechanism of HLJDD against <jats:italic>C. albicans<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2016\/3198249","type":"journal-article","created":{"date-parts":[[2016,4,7]],"date-time":"2016-04-07T00:35:34Z","timestamp":1459989334000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Transcriptomics Analysis of <i>Candida albicans<\/i> Treated with Huanglian Jiedu Decoction Using RNA\u2010seq"],"prefix":"10.1155","volume":"2016","author":[{"given":"Qianqian","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maocan","family":"Tao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,6]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0190-2013"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609370104"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(03)47003-3"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00517-08"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00587-10"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-9-1901"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/18.2.240"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.382"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.7.1482"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.41.12.5623-5632.2003"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt330"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120452"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0925-4439(02)00087-x"},{"key":"e_1_2_9_14_2","series-title":"Lecture Notes in Mathematics","volume-title":"Analyse Harmonique Non-Commutative sur Certains Espaces Homogenes","author":"Coifman R. 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