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Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF) is an important regulator for differentiation.<jats:italic>Methods<\/jats:italic>. To explore the effect of miR-146a\/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI) was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a\/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL LPS treatment were determined.<jats:italic>Results<\/jats:italic>. PEG-PEI prepared at N\/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N\/P ratio increased. Drug release rate amounted to 70% at the first 12\u2009h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a\/bFGF gel group.<jats:italic>Conclusions<\/jats:italic>. PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs.<\/jats:p>","DOI":"10.1155\/2016\/3892685","type":"journal-article","created":{"date-parts":[[2016,1,24]],"date-time":"2016-01-24T16:01:47Z","timestamp":1453651307000},"page":"1-12","source":"Crossref","is-referenced-by-count":9,"title":["Effect of miR-146a\/bFGF\/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells"],"prefix":"10.1155","volume":"2016","author":[{"given":"Lu","family":"Liu","sequence":"first","affiliation":[{"name":"Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Shu","sequence":"additional","affiliation":[{"name":"Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary Shunpan","family":"Cheung","sequence":"additional","affiliation":[{"name":"Conservative Dentistry, School of Dentistry, The University of Hong Kong, Pok Fu Lam, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wei","sequence":"additional","affiliation":[{"name":"Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, 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