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After injections, animals were organized into two groups: NBH (<jats:italic>n<\/jats:italic> = 26) and ME7 (<jats:italic>n<\/jats:italic> = 29). At 15th week after injections (wpi), animals were challenged intranasally with a suspension of Piry arbovirus 0.001% or with NBH. Behavioral changes in ME7 animals appeared in burrowing activity at 14\u2009wpi. Hyperactivity on open field test, errors on rod bridge, and time reduction in inverted screen were detected at 15th, 19th, and 20th wpi respectively. Burrowing was more sensitive to earlier hippocampus dysfunction. However, Piry\u2010infection did not significantly affect the already ongoing burrowing decline in the ME7\u2010treated mice. After behavioral tests, brains were processed for IBA1, protease\u2010resistant form of PrP, and Piry virus antigens. Although virus infection in isolation did not change the number of microglia in CA1, virus infection in prion diseased mice (at 17th wpi) induced changes in number and morphology of microglia in a laminar\u2010dependent way. We suggest that virus infection exacerbates microglial inflammatory response to a greater degree in prion\u2010infected mice, and this is not necessarily correlated with hippocampal\u2010dependent behavioral deficits.<\/jats:p>","DOI":"10.1155\/2016\/3974648","type":"journal-article","created":{"date-parts":[[2016,11,27]],"date-time":"2016-11-27T21:01:11Z","timestamp":1480280471000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Virus Infections on Prion Diseased Mice Exacerbate Inflammatory Microglial Response"],"prefix":"10.1155","volume":"2016","author":[{"given":"Nara","family":"Lins","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz","family":"Mour\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nonata","family":"Tr\u00e9via","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Passos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Augusto","family":"Farias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarila","family":"Assun\u00e7\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9567-0829","authenticated-orcid":false,"given":"Amanda","family":"Quintairos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o","family":"Bento-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia","family":"Consentino Kronka Sosthenes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Antonio Pican\u00e7o","family":"Diniz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Fernando da Costa","family":"Vasconcelos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6611-6880","authenticated-orcid":false,"given":"Cristovam Wanderley","family":"Pican\u00e7o-Diniz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,27]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2012.10.006"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-7634(88)80004-6"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0166-2236(00)02088-9"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/archpsyc.58.5.445"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1385\/jmn:21:3:199"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2005.04.010"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2004.10.003"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-73"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2012.02.003"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280591002450"},{"key":"e_1_2_6_11_2","first-page":"451","article-title":"Altera\u00e7\u00f5es ultraestrutu-rais na infec\u00e7\u00e3o experimental com arbov\u00edrus na Amaz\u00f4nia. 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