{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:29:44Z","timestamp":1776904184971,"version":"3.51.2"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T00:00:00Z","timestamp":1451347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["2015\/21101-5"],"award-info":[{"award-number":["2015\/21101-5"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["2012\/14342-8"],"award-info":[{"award-number":["2012\/14342-8"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The State of S\u00e3o Paulo Research Foundation","award":["2015\/21101-5"],"award-info":[{"award-number":["2015\/21101-5"]}]},{"name":"The State of S\u00e3o Paulo Research Foundation","award":["2012\/14342-8"],"award-info":[{"award-number":["2012\/14342-8"]}]},{"name":"The State of S\u00e3o Paulo Research Foundation","award":["2015\/21101-5"],"award-info":[{"award-number":["2015\/21101-5"]}]},{"name":"The State of S\u00e3o Paulo Research Foundation","award":["2012\/14342-8"],"award-info":[{"award-number":["2012\/14342-8"]}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["2015\/21101-5"],"award-info":[{"award-number":["2015\/21101-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["2012\/14342-8"],"award-info":[{"award-number":["2012\/14342-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>Arsenic trioxide (As<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>) has shown effectiveness in treatment of leukemia but is also associated with reproductive toxicity. Since remediation with N\u2010acetylcysteine (NAC) may mitigate the adverse effects caused by exposure, we assessed the effects of As<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>and its potential reversibility after exposure cessation or coadministration of NAC. Animals received 0.3 or 3.0\u2009mg\/Kg\/day of As<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>subcutaneously and 40\u2009mM of NAC in tap water. As<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>treatment impaired spermatogenesis and sperm motility and decreased seminal vesicle weight and testosterone serum levels; after suspension of treatment, these parameters remained altered. When NAC was administered, animals showed improvement in sperm parameters and seminal vesicle weight.<jats:italic>In vitro<\/jats:italic>epididymal contractility was increased in As<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>\u2010treated animals. We concluded that As<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>is toxic to the male mouse genital system by compromising sperm quality and quantity; these effects persisted even after suspension of the treatment. However, the coadministration of NAC ameliorates the harmful effects of the drug on the male genital system.<\/jats:p>","DOI":"10.1155\/2016\/4257498","type":"journal-article","created":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T21:02:31Z","timestamp":1451422951000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["The Coadministration of N\u2010Acetylcysteine Ameliorates the Effects of Arsenic Trioxide on the Male Mouse Genital System"],"prefix":"10.1155","volume":"2016","author":[{"given":"Raquel Frenedoso","family":"da Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cibele dos Santos","family":"Borges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia","family":"Villela e Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8686-144X","authenticated-orcid":false,"given":"Gabriela","family":"Missassi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Ricardo Almeida","family":"Kiguti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Sampaio","family":"Pupo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Barbosa Junior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janete Aparecida","family":"Anselmo-Franci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilma De Grava","family":"Kempinas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,29]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0039-9140(02)00268-0"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/pmj.79.933.391"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.5.1701"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/nejm199811053391901"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.18.3852"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-08-3532"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.4084\/MJHID.2011.056"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-013-1791-4"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400527"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx700366x"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2011.04.013"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0899-9007(97)00338-9"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9574lsf"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.014"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0540103"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1320"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00153"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2009.0860"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0273-2300(03)00026-6"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2012.05.011"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2010.04.001"},{"key":"e_1_2_8_22_2","first-page":"1263","volume-title":"Principles and Methods of Toxicology","author":"Clegg E. D.","year":"2001"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1159\/000147794"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)88938-4"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2011.04.087"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-002-0095-5"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.37.1.397"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/em.21919"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/617202"},{"key":"e_1_2_8_30_2","first-page":"739","article-title":"The apoptotic mechanism of hepatocellular carcinoma cell line (HepG2) induced by arsenic trioxide","volume":"45","author":"Zhao W.","year":"2014","journal-title":"Journal of Sichuan University: Medical Science Edition"},{"key":"e_1_2_8_31_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0031-6997(25)06663-3","article-title":"Nitric oxide: physiology, pathophysiology, and pharmacology","volume":"43","author":"Moncada S.","year":"1991","journal-title":"Pharmacological Reviews"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0891-5849(96)00275-4"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208484"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1023\/a:1015331229263"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290206"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.04.008"},{"key":"e_1_2_8_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s005990050063"},{"key":"e_1_2_8_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1649"},{"key":"e_1_2_8_39_2","volume-title":"Histopathology of Preclinical Toxicity Studies: Interpretation and Relevance in Drug Safety Evaluation","author":"Greaves P.","year":"2000"},{"key":"e_1_2_8_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2013.09.003"},{"key":"e_1_2_8_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2006.02.002"},{"key":"e_1_2_8_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2006.12.029"},{"key":"e_1_2_8_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2011.08.145"},{"key":"e_1_2_8_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105890"},{"key":"e_1_2_8_45_2","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.111.013466"},{"key":"e_1_2_8_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2008.03.019"},{"key":"e_1_2_8_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066091"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2016\/4257498.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2016\/4257498.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2016\/4257498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T23:35:57Z","timestamp":1748734557000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2016\/4257498"}},"subtitle":[],"editor":[{"given":"Joseph A.","family":"Adeyemi","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,12,29]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1155\/2016\/4257498"],"URL":"https:\/\/doi.org\/10.1155\/2016\/4257498","archive":["Portico"],"relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,29]]},"assertion":[{"value":"2015-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"4257498"}}