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We found that SPRC significantly reduced hepatic reactive oxygen species (ROS) and methane dicarboxylic aldehyde (MDA) levels. Moreover, SPRC also increased the superoxide dismutase (SOD) activity. By Western blot, we found that this protective effect of SPRC was importantly attributed to the regulated hepatic antioxidant\u2010related proteins, including protein kinase B (Akt), heme oxygenase\u20101 (HO\u20101), nuclear factor erythroid 2\u2010related factor 2 (Nrf2), and cystathionine <jats:italic>\u03b3<\/jats:italic>\u2010lyase (CSE, an enzyme that synthesizes hydrogen sulfide). Next, we examined the detailed molecular mechanism of the SPRC protective effect using oleic acid\u2010 (OA\u2010) induced HepG2 cells. The results showed that SPRC significantly decreased intracellular ROS and MDA levels in OA\u2010induced HepG2 cells by upregulating the phosphorylation of Akt, the expression of HO\u20101 and CSE, and the translocation of Nrf2. SPRC\u2010induced HO\u20101 expression and Nrf2 translocation were abolished by the phosphoinositide 3\u2010kinase (PI3K) inhibitor LY294002. Moreover, the antioxidative effect of SPRC was abolished by CSE inhibitor DL\u2010propargylglycine (PAG) and HO\u20101 siRNA. Therefore, these results proved that SPRC produced an antioxidative effect on NAFLD through the PI3K\/Akt\/Nrf2\/HO\u20101 signaling pathway.<\/jats:p>","DOI":"10.1155\/2016\/4690857","type":"journal-article","created":{"date-parts":[[2016,5,23]],"date-time":"2016-05-23T17:11:01Z","timestamp":1464023461000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["S\u2010Propargyl\u2010cysteine Exerts a Novel Protective Effect on Methionine and Choline Deficient Diet\u2010Induced Fatty Liver via Akt\/Nrf2\/HO\u20101 Pathway"],"prefix":"10.1155","volume":"2016","author":[{"given":"Wenwen","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenfen","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laiyin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuilan","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichun","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YiZhun","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,22]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2007.04824.x"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.3897"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0016-5085(98)70599-2"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2011.04.013"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42488-4"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2.10.3290025"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-011-8181-5"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfg083"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m309660200"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2008.08.007"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2011.12.059"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/fjc.0b013e3181ac8e12"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2947"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2010.09.001"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5449"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.05.001"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4944"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2012.01949.x"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10809"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26461"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22039"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.01.025"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/758358"},{"key":"e_1_2_9_24_2","first-page":"95","article-title":"Quantification and mechanisms of oleic acid-induced steatosis in HepG2 cells","volume":"2","author":"Cui W.","year":"2010","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI82204"},{"key":"e_1_2_9_27_2","first-page":"67","article-title":"Comparative effect of pioglitazone, quercetin and hydroxy citric acid on the status of lipid peroxidation and antioxidants in experimental non-alcoholic steatohepatitis","volume":"65","author":"Surapaneni K. 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