{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:44:18Z","timestamp":1740170658717,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["N N402 096 32\/3258","N N402 468937","N N401 293939","K\/ZDS\/001488"],"award-info":[{"award-number":["N N402 096 32\/3258","N N402 468937","N N401 293939","K\/ZDS\/001488"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["N N402 096 32\/3258","N N402 468937","N N401 293939","K\/ZDS\/001488"],"award-info":[{"award-number":["N N402 096 32\/3258","N N402 468937","N N401 293939","K\/ZDS\/001488"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["N N402 096 32\/3258","N N402 468937","N N401 293939","K\/ZDS\/001488"],"award-info":[{"award-number":["N N402 096 32\/3258","N N402 468937","N N401 293939","K\/ZDS\/001488"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009045","name":"Jagiellonian University Medical College","doi-asserted-by":"crossref","award":["N N402 096 32\/3258","N N402 468937","N N401 293939","K\/ZDS\/001488"],"award-info":[{"award-number":["N N402 096 32\/3258","N N402 468937","N N401 293939","K\/ZDS\/001488"]}],"id":[{"id":"10.13039\/100009045","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. Products of angiotensin (ANG) I metabolism may predispose to vascular complications of diabetes mellitus.<jats:italic> Methods<\/jats:italic>. Diabetes was induced with streptozotocin (75\u2009mg\/kg i.p.). Rat aorta fragments, isolated 4 weeks later, were pretreated with perindoprilat (3\u2009<jats:italic>\u03bc<\/jats:italic>M), thiorphan (3\u2009<jats:italic>\u03bc<\/jats:italic>M), or vehicle and incubated for 15 minutes with ANG I (1 <jats:italic>\u03bc<\/jats:italic>M). Products of ANG I metabolism through classical (ANG II, ANG III, and ANG IV) and alternative (ANG (1\u20139), ANG (1\u20137), and ANG (1\u20135)) pathways were measured in the buffer, using liquid chromatography-mass spectrometry.<jats:italic> Results<\/jats:italic>. Incubation with ANG I resulted in higher concentration of ANG II (<jats:italic>P<\/jats:italic> = 0.02, vehicle pretreatment) and lower of ANG (1\u20139) (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.048<\/mml:mn><\/mml:math>, perindoprilat pretreatment) in diabetes. Preference for the classical pathway is suggested by higher ANG III\/ANG (1\u20137) ratios in vehicle (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.03<\/mml:mn><\/mml:math>), perindoprilat (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.02<\/mml:mn><\/mml:math>), and thiorphan pretreated (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.02<\/mml:mn><\/mml:math>) diabetic rat. Within the classical pathway, ratios of ANG IV\/ANG II (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><\/mml:math>) and of ANG IV\/ANG III (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.049<\/mml:mn><\/mml:math>), but not of ANG III\/ANG II are lower in diabetes. <jats:italic>Conclusions<\/jats:italic>. Diabetes in rats led to preference toward deleterious (ANG II, ANG III) over protective (ANG IV, ANG (1\u20139), and ANG (1\u20137)) ANG I metabolites.<\/jats:p>","DOI":"10.1155\/2016\/4846819","type":"journal-article","created":{"date-parts":[[2016,10,10]],"date-time":"2016-10-10T17:01:40Z","timestamp":1476118900000},"page":"1-10","source":"Crossref","is-referenced-by-count":0,"title":["Exogenous Angiotensin I Metabolism in Aorta Isolated from Streptozotocin Treated Diabetic Rats"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9322-5545","authenticated-orcid":true,"given":"P. 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University Medical College, Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Madej","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Jagiellonian University Medical College, Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Rutowski","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Medical Faculty, University of Rzesz\u00f3w, Rzesz\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Korbut","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Jagiellonian University Medical College, Krakow, 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