{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:22:13Z","timestamp":1790392933825,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 11220100100032"],"award-info":[{"award-number":["PIP 11220100100032"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2016]]},"abstract":"CD4+ T follicular helper cells (T<\/mml:mi><\/mml:mrow>F<\/mml:mi>H<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>) were assessed in adult patients with common variable immune deficiency (CVID) classified according to the presence of granulomatous disease (GD), autoimmunity (AI), or both GD and AI (Group I) or the absence of AI and GD (Group II).T<\/mml:mi><\/mml:mrow>F<\/mml:mi>H<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>lymphocytes were characterized by expression of CXCR5 and PD-1.T<\/mml:mi><\/mml:mrow>F<\/mml:mi>H<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>were higher (in both absolute number and percentage) in Group I than in Group II CVID patients and normal controls (N). Within CXCR5+CD4+ T cells, the percentage of PD-1 (+) was higher and that of CCR7 (+) was lower in Group I than in Group II and N. The percentages of Treg andT<\/mml:mi><\/mml:mrow>F<\/mml:mi>H<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>reg were similar in both CVID groups and in N.T<\/mml:mi><\/mml:mrow>F<\/mml:mi>H<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>responded to stimulation increasing the expression of the costimulatory molecules CD40L and ICOS as did N. After submitogenic PHA+IL-2 stimulation, intracellular expression ofT<\/mml:mi><\/mml:mrow>F<\/mml:mi>H<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>cytokines (IL-10, IL-21) was higher than N in Group I, and IL-4 was higher than N in Group II. These results suggest thatT<\/mml:mi><\/mml:mrow>F<\/mml:mi>H<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>are functional in CVID and highlight the association of increased circulatingT<\/mml:mi><\/mml:mrow>F<\/mml:mi>H<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>with AI and GD manifestations.<\/jats:p>","DOI":"10.1155\/2016\/4951587","type":"journal-article","created":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T21:02:42Z","timestamp":1458162162000},"page":"1-10","source":"Crossref","is-referenced-by-count":36,"title":["Common Variable Immunodeficiency and Circulating TFH<\/sub>"],"prefix":"10.1155","volume":"2016","author":[{"given":"Ana","family":"Coraglia","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Hematol\u00f3gicas (IIHEMA), Academia Nacional de Medicina, C1425ASU Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Galassi","sequence":"additional","affiliation":[{"name":"IMEX-CONICET, Academia Nacional de Medicina, C1425ASU Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego S.","family":"Fern\u00e1ndez Romero","sequence":"additional","affiliation":[{"name":"Unidad de Alergia, Asma e Inmunolog\u00eda Cl\u00ednica, C1035AAT Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Cecilia","family":"Juri","sequence":"additional","affiliation":[{"name":"Unidad de Alergia, Asma e Inmunolog\u00eda Cl\u00ednica, C1035AAT Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Felippo","sequence":"additional","affiliation":[{"name":"IMEX-CONICET, Academia Nacional de Medicina, C1425ASU Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Malbr\u00e1n","sequence":"additional","affiliation":[{"name":"Unidad de Alergia, Asma e Inmunolog\u00eda Cl\u00ednica, C1035AAT Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda M. E. de","family":"Bracco","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Hematol\u00f3gicas (IIHEMA), Academia Nacional de Medicina, C1425ASU Ciudad Aut\u00f3noma de Buenos Aires, Argentina"},{"name":"IMEX-CONICET, Academia Nacional de Medicina, C1425ASU Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1006\/clim.1999.4799"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1999.00109.x"},{"key":"3","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1016\/S1081-1206(10)61142-8","volume":"94","year":"2005","journal-title":"Annals of Allergy, Asthma & Immunology"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-013-9901-6"},{"issue":"1","key":"5","first-page":"31","volume":"86","year":"1993","journal-title":"Quarterly Journal of Medicine"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1006\/clim.1999.4725"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-007-9075-1"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1086\/587669"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-124545"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1038\/nri3466"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3233\/jpi-2011-0320"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1097\/00130832-200312000-00001"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa051568"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364431"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1172\/JCI40231"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1172\/jci39748"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903543106"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1038\/ng1600"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1038\/ng1601"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1038\/ng0407-429"},{"issue":"9","key":"21","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1182\/blood-2008-02-141937","volume":"113","year":"2009","journal-title":"Blood"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2009.07.003"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-011-9613-8"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.12.012"},{"issue":"7","key":"25","doi-asserted-by":"crossref","first-page":"4927","DOI":"10.4049\/jimmunol.177.7.4927","volume":"177","year":"2006","journal-title":"The Journal of Immunology"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1002\/art.25032"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.26.021607.090331"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1679"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000209855.28282.ce"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1877"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003161"},{"issue":"4","key":"32","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.immuni.2013.09.007","volume":"39","year":"2013","journal-title":"Immunity"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2425"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-10-3994"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.2001.1802"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2011.09.007"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.11.014"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-06-091744"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.5.1544"},{"issue":"32","key":"40","doi-asserted-by":"crossref","first-page":"13451","DOI":"10.1073\/pnas.0901984106","volume":"106","year":"2009","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-09-243071"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1038\/nature04444"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2496"},{"issue":"2","key":"45","first-page":"174","volume":"110","year":"1997","journal-title":"Clinical and Experimental Immunology"},{"issue":"12","key":"46","doi-asserted-by":"crossref","first-page":"4234","DOI":"10.1182\/blood.V84.12.4234.bloodjournal84124234","volume":"84","year":"1994","journal-title":"Blood"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1997.d01-989.x"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100152"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2010.18"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20081886"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01122.x"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1002\/art.25045"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-06-583260"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141748"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2016\/4951587.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2016\/4951587.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2016\/4951587.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T07:13:07Z","timestamp":1600326787000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/jir\/2016\/4951587\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":53,"alternative-id":["4951587","4951587"],"URL":"https:\/\/doi.org\/10.1155\/2016\/4951587","relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}