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We examined the effects of eMPs on cultured endothelial cells (ECs) and isolated vessels and investigated the role of eMP\u2010derived reactive oxygen species (ROS) and redox signaling in these processes. eMPs were isolated from EC media and their ability to directly produce ROS was assessed by lucigenin and liquid chromatography. Nicotinamide adenine dinucleotide phosphate oxidase (Nox) subunits were probed by Western blot. ECs were treated with eMPs and effects on kinase signaling, superoxide anion () generation, and nitric oxide (NO) production were examined. Acetylcholine\u2010mediated vasorelaxation was assessed by myography in eMP\u2010treated mesenteric arteries. eMPs contained Nox1, Nox2, Nox4, p47\n                    <jats:sup>phox<\/jats:sup>\n                    , p67\n                    <jats:sup>phox<\/jats:sup>\n                    , and p22\n                    <jats:sup>phox<\/jats:sup>\n                    and they produced ROS which was inhibited by the Nox inhibitor, apocynin. eMPs increased phosphorylation of ERK1\/2 and Src, increased  production, and decreased A23187\u2010induced NO production in ECs. Pretreatment of eMPs with apocynin diminished eMP\u2010mediated effects on ROS and NO production but had no effect on eMP\u2010mediated kinase activation or impairment in vasorelaxation. Our findings identify a novel mechanism whereby eMP\u2010derived ROS contributes to MP bioactivity. These interactions may be important in conditions associated with vascular injury and increased eMP formation.\n                  <\/jats:p>","DOI":"10.1155\/2016\/5047954","type":"journal-article","created":{"date-parts":[[2016,5,23]],"date-time":"2016-05-23T17:03:12Z","timestamp":1464022992000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Endothelial Microparticle\u2010Derived Reactive Oxygen Species: Role in Endothelial Signaling and Vascular Function"],"prefix":"10.1155","volume":"2016","author":[{"given":"Dylan","family":"Burger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maddison","family":"Turner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes N.","family":"Munkonda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhian 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