{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T06:12:33Z","timestamp":1764655953433,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370989","81570821","2013CB967004","20081148"],"award-info":[{"award-number":["81370989","81570821","2013CB967004","20081148"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370989","81570821","2013CB967004","20081148"],"award-info":[{"award-number":["81370989","81570821","2013CB967004","20081148"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["81370989","81570821","2013CB967004","20081148"],"award-info":[{"award-number":["81370989","81570821","2013CB967004","20081148"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shandong Academy of Medical Sciences","award":["81370989","81570821","2013CB967004","20081148"],"award-info":[{"award-number":["81370989","81570821","2013CB967004","20081148"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Purpose.<\/jats:italic>To evaluate the changes of keratocytes and dendritic cells in the central clear graft by laser scanning in vivo confocal microscopy after penetrating keratoplasty (PK).<jats:italic>Methods.<\/jats:italic>Thirty adult subjects receiving PK at Shandong Eye Institute and with clear grafts and no sign of immune rejection after surgery were recruited into this study, and 10 healthy adults were controls. The keratocytes and dendritic cells in the central graft were evaluated by laser scanning confocal microscopy, as well as epithelium cells, keratocytes, corneal endothelium cells, and corneal nerves (especially subepithelial plexus nerves).<jats:italic>Results.<\/jats:italic>Median density of subepithelial plexus nerves, keratocyte density in each layer of the stroma, and density of corneal endothelium cells were all lower in clear grafts than in controls. The dendritic cells of five (16.7%) patients were active in Bowman\u2019s membrane and stromal membrane of the graft after PK.<jats:italic>Conclusions.<\/jats:italic>Activated dendritic cells and Langerhans cells could be detected in some of the clear grafts, which indicated that the subclinical stress of immune reaction took part in the chronic injury of the clear graft after PK, even when there was no clinical rejection episode.<\/jats:p>","DOI":"10.1155\/2016\/5159746","type":"journal-article","created":{"date-parts":[[2016,2,29]],"date-time":"2016-02-29T21:01:44Z","timestamp":1456779704000},"page":"1-6","source":"Crossref","is-referenced-by-count":6,"title":["Laser Scanning In Vivo Confocal Microscopy of Clear Grafts after Penetrating Keratoplasty"],"prefix":"10.1155","volume":"2016","author":[{"given":"Dai","family":"Wang","sequence":"first","affiliation":[{"name":"Shandong Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Jinan 250012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Song","sequence":"additional","affiliation":[{"name":"Qingdao Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuting","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Jinan 250012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dapeng","family":"Sun","sequence":"additional","affiliation":[{"name":"Qingdao Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuexin","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Jinan 250012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qingdao Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao 266071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1326-6946","authenticated-orcid":true,"given":"Hua","family":"Gao","sequence":"additional","affiliation":[{"name":"Shandong Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Jinan 250012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"6","key":"1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.5694\/j.1326-5377.1992.tb137253.x","volume":"157","year":"1992","journal-title":"The Medical Journal of Australia"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2004.00417.x"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1097\/01.icl.0000062460.03555.32"},{"issue":"1","key":"4","first-page":"110","volume":"115","year":"2002","journal-title":"Chinese Medical Journal"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200201000-00017"},{"issue":"2","key":"6","first-page":"157","volume":"14","year":"1995","journal-title":"Cornea"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1080\/02713680500320752"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-1425"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1007\/s004170100285"},{"issue":"4","key":"10","first-page":"307","volume":"43","year":"2007","journal-title":"Chinese Journal of Ophthalmology"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200001000-00009"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000154384.05614.8f"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.2009.02016.x"},{"issue":"1","key":"14","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1136\/bjophthalmol-2015-306934","volume":"100","year":"2016","journal-title":"The British Journal of Ophthalmology"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2013.842592"},{"issue":"2","key":"16","first-page":"65","volume":"22","year":"2006","journal-title":"Yan Ke Xue Bao"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200108000-00002"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2005.09.006"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-010-1403-0"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-1255"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041630108"},{"issue":"3","key":"22","first-page":"487","volume":"39","year":"1998","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.116.11.1425"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0561"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2006.01.048"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(03)46023-8"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200201000-00013"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1983.01040020389010"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200308000-00005"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1046\/j.1475-1313.1999.00408.x"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-4835(03)00050-2"},{"issue":"6","key":"32","first-page":"1100","volume":"36","year":"1995","journal-title":"Investigative Ophthalmology & Visual Science"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2016\/5159746.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2016\/5159746.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2016\/5159746.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T07:08:43Z","timestamp":1600240123000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2016\/5159746\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":32,"alternative-id":["5159746","5159746"],"URL":"https:\/\/doi.org\/10.1155\/2016\/5159746","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2016]]}}}