{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T12:14:05Z","timestamp":1768479245975,"version":"3.49.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20GM103480"],"award-info":[{"award-number":["P20GM103480"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>Use of antioxidants to mitigate oxidative stress during ocular inflammatory diseases has shown therapeutic potential. This work examines a nanoscale therapeutic modality for the eye on the base of antioxidant enzyme, superoxide dismutase 1 (SOD1), termed \u201cnanozyme.\u201d The nanozyme is produced by electrostatic coupling of the SOD1 with a cationic block copolymer, poly(L\u2010lysine)\u2010poly(ethyleneglycol), followed by covalent cross\u2010linking of the complexes with 3,3\u2032\u2010dithiobis(sulfosuccinimidylpropionate) sodium salt. The ability of SOD1 nanozyme as well as the native SOD1 to reduce inflammatory processes in the eye was examined <jats:italic>in vivo<\/jats:italic> in rabbits with immunogenic uveitis. Results suggested that topical instillations of both enzyme forms demonstrated anti\u2010inflammatory activity; however, the nanozyme was much more effective compared to the free enzyme in decreasing uveitis manifestations. In particular, we noted statistically significant differences in such inflammatory signs in the eye as the intensities of corneal and iris edema, hyperemia of conjunctiva, lens opacity, fibrin clots, and the protein content in aqueous humor. Clinical findings were confirmed by histological data. Thus, SOD1\u2010containing nanozyme is potentially useful therapeutic agent for the treatment of ocular inflammatory disorders.<\/jats:p>","DOI":"10.1155\/2016\/5194239","type":"journal-article","created":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T21:02:50Z","timestamp":1449003770000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Superoxide Dismutase 1 Nanozyme for Treatment of Eye Inflammation"],"prefix":"10.1155","volume":"2016","author":[{"given":"Olga A.","family":"Kost","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga V.","family":"Beznos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina G.","family":"Davydova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devika S.","family":"Manickam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina I.","family":"Nikolskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna E.","family":"Guller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr V.","family":"Binevski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia B.","family":"Chesnokova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anatoly B.","family":"Shekhter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia L.","family":"Klyachko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander V.","family":"Kabanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.5301\/ejo.5000278"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.58471"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/09273948.2012.723112"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004397-200201000-00018"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.3129\/i10-081"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000336183"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/10807680151125537"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000265413"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0748-5514(86)80059-9"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1981.03930010886020"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1159\/000267648"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000266813"},{"key":"e_1_2_8_13_2","first-page":"886","article-title":"Role of oxygen radicals in experimental allergic uveitis","volume":"28","author":"Rao N. 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