{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:58:20Z","timestamp":1792623500331,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["WT 087546MA"],"award-info":[{"award-number":["WT 087546MA"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["WT 087546MA"],"award-info":[{"award-number":["WT 087546MA"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>The frequent administering of antibiotics in the treatment of poultry diseases may contribute to emergence of antimicrobial-resistant strains. The objective of this study was to detect the presence of extended-spectrum<jats:italic>\u03b2<\/jats:italic>-lactamase- (ESBL-) producing<jats:italic>Escherichia coli<\/jats:italic>in poultry in Zambia. A total of 384 poultry samples were collected and analyzed for ESBL-producing<jats:italic>Escherichia coli<\/jats:italic>. The cultured<jats:italic>E. coli<\/jats:italic>isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction for detection of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:msub><mml:mrow><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>CTX-M<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:msub><mml:mrow><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>SHV<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:math>, and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:msub><mml:mrow><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>TEM<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:math>genes. Overall 20.1%, 77\/384, (95% CI; 43.2\u201365.5%) of total samples analyzed contained ESBL-producing<jats:italic>Escherichia coli<\/jats:italic>. The antimicrobial sensitivity test revealed that 85.7% (66\/77; CI: 75.7\u201392) of ESBL-producing<jats:italic>E. coli<\/jats:italic>isolates conferred resistance to beta-lactam and other antimicrobial agents. These results indicate that poultry is a potential reservoir for ESBL-producing<jats:italic>Escherichia coli<\/jats:italic>. The presence of ESBL-producing<jats:italic>Escherichia coli<\/jats:italic>in poultry destined for human consumption requires strengthening of the antibiotic administering policy. This is important as antibiotic administration in food animals is gaining momentum for improved animal productivity in developing countries such as Zambia.<\/jats:p>","DOI":"10.1155\/2016\/5275724","type":"journal-article","created":{"date-parts":[[2016,4,17]],"date-time":"2016-04-17T17:01:23Z","timestamp":1460912483000},"page":"1-5","source":"Crossref","is-referenced-by-count":57,"title":["Detection of Extended-Spectrum Beta-Lactamase-Producing<i>Escherichia coli<\/i>in Market-Ready Chickens in Zambia"],"prefix":"10.1155","volume":"2016","author":[{"given":"K.","family":"Chishimba","sequence":"first","affiliation":[{"name":"Lusaka City Council, Public Health Department, P.O. Box 30789, Lusaka, Zambia"},{"name":"Microbiology Unit, Paraclinical Studies, University of Zambia, P.O. Box 32379, Lusaka, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. M.","family":"Hang\u2019ombe","sequence":"additional","affiliation":[{"name":"Microbiology Unit, Paraclinical Studies, University of Zambia, P.O. Box 32379, Lusaka, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Muzandu","sequence":"additional","affiliation":[{"name":"Microbiology Unit, Paraclinical Studies, University of Zambia, P.O. Box 32379, Lusaka, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. E.","family":"Mshana","sequence":"additional","affiliation":[{"name":"Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. I.","family":"Matee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, Muhimbili University of Health and Allied Sciences, P.O. Box 65001, Dar es Salaam, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan"},{"name":"The Global Station for Zoonosis Control, Hokkaido University, Sapporo, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan"},{"name":"The Global Station for Zoonosis Control, Hokkaido University, Sapporo, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","year":"2014"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks065"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01851.x"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/515689"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1128\/aac.47.6.2056-2058.2003"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq396"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00262-10"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn093"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.168.17.1897"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-2-49"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-12-28"},{"key":"12","year":"2005"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.03.009"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1128\/aac.49.4.1319-1322.2005"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028x.jfp-14-445"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.3201\/eid1908.120879"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-1-74"},{"key":"18","series-title":"19th Informational Supplement M100-S19","year":"2009"}],"container-title":["International Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2016\/5275724.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2016\/5275724.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijmicro\/2016\/5275724.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T16:29:37Z","timestamp":1471537777000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/ijmicro\/2016\/5275724\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":18,"alternative-id":["5275724","5275724"],"URL":"https:\/\/doi.org\/10.1155\/2016\/5275724","relation":{},"ISSN":["1687-918X","1687-9198"],"issn-type":[{"value":"1687-918X","type":"print"},{"value":"1687-9198","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}