{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T01:13:38Z","timestamp":1778980418141,"version":"3.51.4"},"reference-count":63,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370487"],"award-info":[{"award-number":["81370487"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. 5-HT enhances dextran sulfate sodium- (DSS-) induced colitis and is involved in inflammatory bowel disease (IBD). Matrix metalloproteinases (MMPs) play roles in the process of intestinal inflammation.<jats:italic>Aims<\/jats:italic>. To examine whether 5-HT induces MMPs expression in mouse colon to enhance DSS-induced colitis.<jats:italic>Materials and Methods<\/jats:italic>. C57BL\/6J (B6) mice were treated with either low-dose (1.0\u2009mg\/kg) or high-dose (2.0\u2009mg\/kg) 5-HT by enema, low-dose (1.0%) or high-dose (2.5%) DSS, or combined low-dose (1.0%) DSS and (1.0\u2009mg\/kg) 5-HT. Mouse colitis was analyzed. MMPs and tissue inhibitors of MMPs (TIMPs) mRNA were measured by real-time quantitative RT-PCR in mouse colon and in human Caco-2 cells and neutrophils. MMP-3 and MMP-9 protein levels were quantified from immunohistochemistry (IHC) images of mouse colons.<jats:italic>Results<\/jats:italic>. 5-HT exacerbated DSS-induced colitis, low-dose 5-HT induces both MMP-3 and MMP-9, and high-dose 5-HT only increased MMP-3 mRNA expression in mouse colon. Mouse colon MMP-3 and MMP-9 protein levels were also elevated by 5-HT treatment. The MMP-2, TIMP-1, and TIMP-2 mRNA levels were increased in the inflamed colon. 5-HT induced MMP-3 and MMP-9 mRNA expression in Caco-2 and human neutrophils, respectively, in vitro.<jats:italic>Conclusion<\/jats:italic>. 5-HT induced MMP-3 and MMP-9 expression in mouse colon; these elevated MMPs may contribute to DSS-induced colitis.<\/jats:p>","DOI":"10.1155\/2016\/5359768","type":"journal-article","created":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T21:12:30Z","timestamp":1467753150000},"page":"1-10","source":"Crossref","is-referenced-by-count":21,"title":["Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1044-396X","authenticated-orcid":true,"given":"Menglu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"},{"name":"Department of Clinical Laboratory, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6562-5296","authenticated-orcid":true,"given":"Pan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yalin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9274-0812","authenticated-orcid":true,"given":"Yongchao","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianjun","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0894-3033","authenticated-orcid":true,"given":"Fong-Fong","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"},{"name":"Department of Cancer Genetics and Epigenetics, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4159-7486","authenticated-orcid":true,"given":"Qiang","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, China"},{"name":"Department of Gastroenterology and Hepatology, Beijing Rehabilitation Hospital of Capital Medical University, 1 Gongliao Road, Shijingshan District, Beijing 100114, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101225"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-012-0352-6"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.01.058"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i1.6"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i1.91"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(03)00556-2"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1960.sp006532"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1126\/science.149.3680.197"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/s0893-133x(99)00031-7"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.105"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/354957"},{"issue":"7","key":"12","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1523\/JNEUROSCI.16-07-02352.1996","volume":"16","year":"1996","journal-title":"The Journal of Neuroscience"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(01)00746-8"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2008.03.020"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.03.013"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2007.02.013"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1078\/0065-1281-00641"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.1.42"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1136\/gut.47.6.804"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.1997.00237.x"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00318.2009"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2014.02.003"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.08.041"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000070112.80711.3d"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.31.21491"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0689-x"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00577.2012"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2015.04.005"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2010.01.003"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-11-233"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2005.02.011"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.103465"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70339-2"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90454.2008"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2010.07920.x"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2007.073809"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2008.7"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1042\/bj2960803"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1159\/000065546"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-011-1936-z"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/964131"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1301906"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.6.3369"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im1525s104"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/102918"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2011.02647.x"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.can-03-2272"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks596"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1007\/bf00712758"},{"issue":"1","key":"50","first-page":"1","volume":"8","year":"1976","journal-title":"Annals of Clinical Research"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-07-2903"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh242"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006453"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2007.07.003"},{"issue":"8","key":"55","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1182\/blood.V96.8.2673","volume":"96","year":"2000","journal-title":"Blood"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.12.009"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.6.4103"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00256.2015"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1097\/01.mib.0000438430.15553.90"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1136\/gut.47.1.63"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.12.006"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10836"},{"issue":"35","key":"63","doi-asserted-by":"crossref","first-page":"25228","DOI":"10.1016\/S0021-9258(19)74029-0","volume":"267","year":"1992","journal-title":"Journal of Biological Chemistry"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2016\/5359768.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2016\/5359768.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2016\/5359768.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T19:14:18Z","timestamp":1656789258000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/mi\/2016\/5359768\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":63,"alternative-id":["5359768","5359768"],"URL":"https:\/\/doi.org\/10.1155\/2016\/5359768","relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}