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MicroRNA\u201021 (miR\u201021) and one of its target proteins, PTEN, contribute to the survival and proliferation of many cell types, but their prosurvival effects in c\u2010kit<jats:sup>+<\/jats:sup> CSC remain unclear. Thus, we hypothesized that miR\u201021 reduces hydrogen peroxide\u2010 (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010) induced apoptosis in c\u2010kit<jats:sup>+<\/jats:sup> CSC and estimated the contribution of PTEN\/PI3K\/Akt signaling to this oxidative circumstance. miR\u201021 mimics efficiently reduced H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced apoptosis in c\u2010kit<jats:sup>+<\/jats:sup> CSC, as evidenced by the downregulation of the proapoptosis proteins caspase\u20103 and Bax and upregulation of the antiapoptotic Bcl\u20102. In addition, the gain of function of miR\u201021 in c\u2010kit<jats:sup>+<\/jats:sup> CSC downregulated the protein level of PTEN although its mRNA level changed slightly; in the meantime, miR\u201021 overexpression also increased phospho\u2010Akt (p\u2010Akt). The antiapoptotic effects of miR\u201021 were comparable with Phen (bpV), the selective inhibitor of PTEN, while miR\u201021 inhibitor or PI3K\u2019s inhibitor LY294002 efficiently attenuated the antiapoptotic effect of miR\u201021. Taken together, these results indicate that the anti\u2010H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced apoptosis effect of miR\u201021 in c\u2010kit<jats:sup>+<\/jats:sup> CSC is contributed by PTEN\/PI3K\/Akt signaling. miR\u201021 could be a potential molecule to facilitate the c\u2010kit<jats:sup>+<\/jats:sup> CSC therapy in ischemic myocardium.<\/jats:p>","DOI":"10.1155\/2016\/5389181","type":"journal-article","created":{"date-parts":[[2016,10,10]],"date-time":"2016-10-10T21:09:42Z","timestamp":1476133782000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["miR\u201021 Reduces Hydrogen Peroxide\u2010Induced Apoptosis in c\u2010kit<sup>+<\/sup> Cardiac Stem Cells In Vitro through PTEN\/PI3K\/Akt 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