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Our study aims to investigate the effect of Salvianolate on rat hepatic injury by protecting hepatocyte mitochondria. To evaluate the effects of Salvianolate on injured hepatocytes, alpha mouse liver 12 (AML\u201012) cells were induced with hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) and treated with Salvianolate. Cell viability and MitoTracker Green for mitochondria and 5,5\u2032,6,6\u2032\u2010tetrachloro\u20101,1\u2032,3,3\u2032\u2010tetraethylbenzimidazole\u2010carbocyanide iodine (JC\u20101) levels and cytochrome C (Cyto\u2010C) expressions were detected\n                    <jats:italic>in vitro<\/jats:italic>\n                    . To identify the effect of Salvianolate on protecting against mitochondria injury, male Wistar rats were injected with carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    ) and treated with Salvianolate (40\u2009mg\u00b7kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). Serum liver function, parameters for peroxidative damage, hematoxylin and eosin (H&amp;E) staining, and transmission electron microscope (TEM) of hepatocyte mitochondria were assayed. Our results showed that Salvianolate effectively protected hepatocytes, increased mitochondria vitality, and decreased Cyto\u2010C expressions\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Besides, Salvianolate alleviated the liver function, attenuated the indicators of peroxidation, and relieved the mitochondria injury\n                    <jats:italic>in vivo<\/jats:italic>\n                    . In conclusion,\n                    <jats:italic>Salvianolate<\/jats:italic>\n                    is effective in protecting hepatocytes from injury\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , and the mechanism might be related to its protective effect on hepatocyte mitochondria against oxidative stress.\n                  <\/jats:p>","DOI":"10.1155\/2016\/5408705","type":"journal-article","created":{"date-parts":[[2016,6,2]],"date-time":"2016-06-02T17:01:45Z","timestamp":1464886905000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Salvianolate Protects Hepatocytes from Oxidative Stress by Attenuating Mitochondrial 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