{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T20:59:49Z","timestamp":1774558789915,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004847","name":"Zahedan University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004847","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Sistan and Baluchestan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Ophthalmology"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. Pterygium is a serious eye problem in countries with high exposure to UV. However, despite numerous studies, the molecular etiology of pterygium is unclear. Recent studies have indicated that<jats:italic>LATS1<\/jats:italic>and<jats:italic>LATS2<\/jats:italic>genes are involved in DDR signaling pathways against continuous UV exposure. Our aim was to evaluate the<jats:italic>LATS1<\/jats:italic>and<jats:italic>LATS2<\/jats:italic>promoter methylation with the risk of pterygium formation.<jats:italic>Methods<\/jats:italic>. We evaluated the promoter methylation status of<jats:italic>LATS1<\/jats:italic>and<jats:italic>LATS2<\/jats:italic>using methylation-specific PCR technique. Also, mRNA expression of<jats:italic>LATS1<\/jats:italic>and<jats:italic>LATS2<\/jats:italic>was assessed in 14 cases of pterygium and 14 normal specimens by real-time PCR.<jats:italic>Results<\/jats:italic>. Promoter methylation of<jats:italic>LATS1<\/jats:italic>and<jats:italic>LATS2<\/jats:italic>was detected significantly between pterygium tissues and normal tissues [<jats:italic>LATS1<\/jats:italic>; OR = 4.9; 95% CI: 1.54 to 15.48,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.003<\/mml:mn><\/mml:math>;<jats:italic>LATS2<\/jats:italic>; OR = 7.1; 95% CI: 1.53 to 33.19,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.004<\/mml:mn><\/mml:math>]. The gene expression analysis showed a statistically significant difference between pterygium tissues and healthy controls for both<jats:italic>LATS1<\/jats:italic>and<jats:italic>LATS2<\/jats:italic>(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>).<jats:italic>Conclusions<\/jats:italic>. The data of this study is the first report regarding the effect of promoter methylation of the<jats:italic>LATS1<\/jats:italic>and<jats:italic>LATS2<\/jats:italic>in the pterygium. To confirm these data, doing further studies in various genetic populations with large sample sizes using advanced molecular techniques is proposed.<\/jats:p>","DOI":"10.1155\/2016\/5431021","type":"journal-article","created":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T16:01:30Z","timestamp":1453996890000},"page":"1-5","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of<i>LATS1<\/i>and<i>LATS2<\/i>Promoter Methylation with the Risk of Pterygium Formation"],"prefix":"10.1155","volume":"2016","author":[{"given":"Maryam","family":"Najafi","sequence":"first","affiliation":[{"name":"Departement of Biology, University of Sistan and Baluchestan, Zahedan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dor Mohammad","family":"Kordi-Tamandani","sequence":"additional","affiliation":[{"name":"Departement of Biology, University of Sistan and Baluchestan, Zahedan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Arish","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Al-Zahra Eye Hospital, Zahedan University of Medical Sciences, Zahedan 98167-43463, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/658648"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1097\/ico.0b013e31823cbecb"},{"issue":"10","key":"3","first-page":"740","volume":"24","year":"2014","journal-title":"Journal of the College of Physicians and Surgeons Pakistan"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.3109\/08820538.2014.971822"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-1773"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.125815"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.072918"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204817"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206603"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/141732"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.113.156372"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205600"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2006.09.006"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1097\/icl.0000000000000126"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9440(10)63850-3"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2011.01020.x"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.16.16873"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1038\/sj\/onc\/1205174"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2007.06.003"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3035"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1447006"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1186\/2001-1326-3-22"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.3892\/ol_00000054"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v19.i27.4363"},{"key":"28","first-page":"1411","volume":"12","year":"2006","journal-title":"Molecular Vision"},{"key":"29","first-page":"1047","volume":"16","year":"2010","journal-title":"Molecular Vision"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014687"}],"container-title":["Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2016\/5431021.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2016\/5431021.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2016\/5431021.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,7,26]],"date-time":"2016-07-26T12:19:11Z","timestamp":1469535551000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/joph\/2016\/5431021\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":28,"alternative-id":["5431021","5431021"],"URL":"https:\/\/doi.org\/10.1155\/2016\/5431021","relation":{},"ISSN":["2090-004X","2090-0058"],"issn-type":[{"value":"2090-004X","type":"print"},{"value":"2090-0058","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}