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Morin, an active ingredient obtained from Moraceae plants, has been demonstrated to have promising anti\u2010inflammatory activities in a range of disorders. However, its impacts on pulmonary diseases, particularly on asthma, have not been clarified. This study was designed to investigate whether morin alleviates airway inflammation in chronic asthma with an emphasis on oxidative stress modulation. <jats:italic>In vivo<\/jats:italic>, ovalbumin\u2010 (OVA\u2010) sensitized mice were administered with morin or dexamethasone before challenge. Bronchoalveolar lavage fluid (BALF) and lung tissues were obtained to perform cell counts, histological analysis, and enzyme\u2010linked immunosorbent assay. <jats:italic>In vitro<\/jats:italic>, human bronchial epithelial cells (BECs) were challenged by tumor necrosis factor alpha (TNF\u2010<jats:italic>\u03b1<\/jats:italic>). The supernatant was collected for the detection of the proinflammatory proteins, and the cells were collected for reactive oxygen species (ROS)\/mitogen\u2010activated protein kinase (MAPK) evaluations. Severe inflammatory responses and remodeling were observed in the airways of the OVA\u2010sensitized mice. Treatment with morin dramatically attenuated the extensive trafficking of inflammatory cells into the BALF and inhibited their infiltration around the respiratory tracts and vessels. Morin administration also significantly suppressed goblet cell hyperplasia and collagen deposition\/fibrosis and dose\u2010dependently inhibited the OVA\u2010induced increases in IgE, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, interleukin\u2010 (IL\u2010) 4, IL\u201013, matrix metalloproteinase\u20109, and malondialdehyde. In human BECs challenged by TNF\u2010<jats:italic>\u03b1<\/jats:italic>, the levels of proteins such as eotaxin\u20101, monocyte chemoattractant protein\u20101, IL\u20108 and intercellular adhesion molecule\u20101, were consistently significantly decreased by morin. Western blotting and the 2\u2032,7\u2032\u2010dichlorofluorescein assay revealed that the increases in intracellular ROS and MAPK phosphorylation were abolished by morin, implying that ROS\/MAPK signaling contributes to the relief of airway inflammation. Our findings indicate for the first time that morin alleviates airway inflammation in chronic asthma, which probably occurs via the oxidative stress\u2010responsive MAPK pathway, highlighting a novel profile of morin as a potent agent for asthma management.<\/jats:p>","DOI":"10.1155\/2016\/5843672","type":"journal-article","created":{"date-parts":[[2015,12,13]],"date-time":"2015-12-13T21:01:07Z","timestamp":1450040467000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["Morin Attenuates Ovalbumin\u2010Induced Airway Inflammation by Modulating Oxidative Stress\u2010Responsive MAPK 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