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The use of this drug has been increasing in clinical practice and intra- or -interindividual variability has been exhibited for special population. For this reason, bioanalytical methods are required for drug monitoring in biological matrices. So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma. However, due to the matrix complexity a previous purification step is required. Unlike other pretreatment techniques presented in the literature, for the first time, a procedure employing ultrafiltration tubes Amicon\u00ae (10\u2009kDa porous size) without organic solvent consumption was developed. GC-MS analyses were carried out using a linear temperature program, capillary fused silica column, and helium as the carrier gas. DLLME optimized parameters were type and volume of extraction and dispersing solvents, salt addition, and vortex agitation time. Under chosen parameters (extraction solvent: chloroform, 130\u2009<jats:italic>\u03bc<\/jats:italic>L; dispersing solvent: isopropyl alcohol, 400\u2009<jats:italic>\u03bc<\/jats:italic>L; no salt addition and no vortex agitation time), the method was completely validated and all parameters were in agreement with the literature recommendations. LEV was quantified in patient\u2019s plasma sample using less than 550\u2009<jats:italic>\u03bc<\/jats:italic>L of organic solvent.<\/jats:p>","DOI":"10.1155\/2016\/5976324","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T17:04:57Z","timestamp":1476723897000},"page":"1-12","source":"Crossref","is-referenced-by-count":10,"title":["Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8437-1268","authenticated-orcid":true,"given":"Greyce Kelly Steinhorst","family":"Alcantara","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, 14040-903 Ribeir\u00e3o Preto, SP, 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