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In spite of the recent progress in targeted therapy, most LADC patients eventually expired due to the inevitable recurrence and drug resistance. New complementary agent with evidence\u2010based molecular mechanism is urgently needed. MiR\u201034a is an important p53 downstream tumor suppressor, which regulates apoptosis, cell\u2010cycle, EMT (epithelial mesenchymal transition), and so forth. Its expression is deficient in many types of cancers including LADC. Here, we show that a Chinese herbal formula JP\u20101 activates p53\/miR\u201034a axis in A549 human LADC cells (p53 wild\u2010type). Treatment with JP\u20101 induces p53 and its downstream p21 and BAX proteins as well as the miR\u201034a, resulting in growth inhibition, colony formation reduction, migration repression, and apoptosis induction. Accordingly, the decreases of miR\u201034a downstream targets such as CDK6, SIRT1, c\u2010Myc, survivin, Snail, and AXL were observed. Moreover, JP\u20101 activates AMPK<jats:italic>\u03b1<\/jats:italic> and reduces mTOR activity, implying its inhibitory effect on the energy\u2010sensitive protein synthesis and cell proliferation signaling. Our results show that JP\u20101 activates p53\/miR\u201034a tumor suppressor axis and decreases proteins related to proliferation, apoptosis resistance, and metastasis, suggesting its potential as a complementary medicine for LADC treatment.<\/jats:p>","DOI":"10.1155\/2016\/5989681","type":"journal-article","created":{"date-parts":[[2016,12,18]],"date-time":"2016-12-18T21:35:39Z","timestamp":1482096939000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Activation of p53\/miR\u201034a Tumor Suppressor Axis by Chinese Herbal Formula JP\u20101 in A549 Lung Adenocarcinoma 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