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We selected a 216\u2009bp short Hsp70B\u2032 core promoter induced by a mutated, constitutively active heat shock factor (mHSF) 1 to drive strong gene expression of therapeutic transgenes MelanA, BclxL, and IL-12p70 in HeLa cells, as well as in mature DCs (mDCs). As this involves overexpressing mHSF1, we first evaluated the resulting effects on DCs regarding upregulation of heat shock proteins and maturation markers, toxicity, cytokine profile, and capacity to induce antigen-specific CD8<jats:sup>+<\/jats:sup>T cells. Second, we generated the two-vector-based \u201cmodular promoter\u201d system, where one vector contains the mHSF1 under the control of the human CD83 promoter, which is specifically active only in DCs and after maturation. mHSF1, in turn, activates the Hsp70B\u2032 core promotor-driven expression of transgenes MelanA and IL-12p70 in the DC-like cell line XS52 and in human mature and hence immunogenic DCs, but not in tolerogenic immature DCs. These<jats:italic>in vitro<\/jats:italic>experiments provide the basis for an<jats:italic>in vivo<\/jats:italic>targeting of mature DCs for the expression of multiple transgenes. Therefore, this modular promoter system represents a promising tool for future DC-based immunotherapies<jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2016\/6078473","type":"journal-article","created":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T19:23:54Z","timestamp":1467228234000},"page":"1-17","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptional Targeting of Mature Dendritic Cells with Adenoviral Vectors via a Modular Promoter System for Antigen Expression and Functional Manipulation"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2866-3901","authenticated-orcid":true,"given":"Ilka","family":"Knippertz","sequence":"first","affiliation":[{"name":"Department of Immune Modulation at the Department of Dermatology, Universit\u00e4tsklinikum Erlangen, Hartmannstrasse 14, 91052 Erlangen, 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