{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:36:42Z","timestamp":1790401002502,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Introduction. Pseudomonas aeruginosa<\/jats:italic>is the most frequently isolated organism as it acts as the opportunistic pathogen and can cause infections in immunosuppressed patients. The production of different types of beta-lactamases renders this organism resistant to many commonly used antimicrobials. Therefore, the aim of this study was to document the antibiotic resistance rate in<jats:italic>Pseudomonas aeruginosa<\/jats:italic>isolated from different clinical specimens.<jats:italic>Methods. Pseudomonas aeruginosa<\/jats:italic>recovered was identified by standard microbiological methods. Antibiotic susceptibility testing was performed by modified Kirby-Bauer disc diffusion method following Clinical and Laboratory Standard Institute (CLSI) guidelines and all the suspected isolates were tested for the production of ESBLs, MBLs, and AmpC.<jats:italic>Results.<\/jats:italic>Out of total (178) isolates, 83.1% were recovered from the inpatient department (IPD). Majority of the isolates mediated resistance towards the beta-lactam antibiotics, while nearly half of the isolates were resistant to ciprofloxacin. Most of the aminoglycosides used showed resistance rate up to 75% but amikacin proved to be better option. No resistance to polymyxin was observed. ESBLs, MBLs, and AmpC mediated resistance was seen in 33.1%, 30.9%, and 15.7% isolates, respectively.<jats:italic>Conclusions<\/jats:italic>. Antibiotic resistance rate and beta-lactamase mediated resistance were high. Thus, regular surveillance of drug resistance is of utmost importance.<\/jats:p>","DOI":"10.1155\/2016\/6437208","type":"journal-article","created":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T21:01:09Z","timestamp":1471986069000},"page":"1-8","source":"Crossref","is-referenced-by-count":8,"title":["Growing Menace of Antibacterial Resistance in Clinical Isolates of<i>Pseudomonas aeruginosa<\/i>in Nepal: An Insight of Beta-Lactamase Production"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1846-1377","authenticated-orcid":true,"given":"Shamshul","family":"Ansari","sequence":"first","affiliation":[{"name":"Department of Microbiology, Chitwan Medical College, Bharatpur, Chitwan, Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabindra","family":"Dhital","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chitwan Medical College, Bharatpur, Chitwan, 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