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To examine the expression patterns of hydrogen sulphide\u2010 (H<jats:sub>2<\/jats:sub>S\u2010) producing enzymes in ischaemic heart tissue and plasma levels of H<jats:sub>2<\/jats:sub>S after 2 weeks of NaHS treatment after myocardial infarction (MI) and to clarify the role of endogenous H<jats:sub>2<\/jats:sub>S in the MI process. <jats:italic>Results<\/jats:italic>. After MI surgery, 2 weeks of treatment with the H<jats:sub>2<\/jats:sub>S donor NaHS alleviated ischaemic injury. Meanwhile, in ischemia myocardium, three H<jats:sub>2<\/jats:sub>S\u2010producing enzymes, cystathionine <jats:italic>\u03b3<\/jats:italic>\u2010lyase (CSE), cystathionine\u2010<jats:italic>\u03b2<\/jats:italic>\u2010synthase (CBS), and 3\u2010mercaptopyruvate sulfurtransferase (3\u2010MST) significantly increased. Plasma H<jats:sub>2<\/jats:sub>S levels were also elevated. <jats:italic>In vitro<\/jats:italic>, NaHS treatment protected cardiomyocytes from hypoxic injury and raised CBS levels in a concentration\u2010dependent manner. Different from <jats:italic>in vivo<\/jats:italic> results, however, CSE or 3\u2010MST expression did not change. NaHS treatment increased the activity of CSE\/CBS but not of 3\u2010MST. When CSE was either knocked down (<jats:italic>in vitro<\/jats:italic>) or knocked out (<jats:italic>in vivo<\/jats:italic>), H<jats:sub>2<\/jats:sub>S levels significantly decreased, which subsequently exacerbated the ischaemic injury. Meanwhile, the expressions of CBS and 3\u2010MST increased due to compensation. <jats:italic>Conclusions<\/jats:italic>. Exogenous H<jats:sub>2<\/jats:sub>S treatment changed the expressions of three H<jats:sub>2<\/jats:sub>S\u2010producing enzymes and H<jats:sub>2<\/jats:sub>S levels after MI, suggesting a new and indirect regulatory mechanism for H<jats:sub>2<\/jats:sub>S production and its contribution to cardiac protection. Endogenous H<jats:sub>2<\/jats:sub>S plays an important role in protecting ischaemic tissue after MI.<\/jats:p>","DOI":"10.1155\/2016\/6492469","type":"journal-article","created":{"date-parts":[[2016,1,5]],"date-time":"2016-01-05T21:05:02Z","timestamp":1452027902000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["The H<sub>2<\/sub>S Donor NaHS Changes the Expression Pattern of H<sub>2<\/sub>S\u2010Producing Enzymes after Myocardial 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