{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:03:30Z","timestamp":1740171810731,"version":"3.37.3"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["388476\/256990"],"award-info":[{"award-number":["388476\/256990"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>There is a need for new noninvasive biomarkers (NIBMs) able to assess cholestasis and fibrosis in chronic cholestatic liver diseases (CCLDs). Tumorigenesis can arise from CCLDs. Therefore, autoantibodies to tumor-associated antigens (TAA) may be early produced in response to abnormal self-antigen expression caused by cholestatic injury. Vascular endothelial growth factor receptor-3 (VEGFR-3) has TAA potential since it is involved in cholangiocytes and lymphatic vessels proliferations during CCLDs. This study aims to detect autoantibodies directed at VEGFR-3 during bile duct ligation- (BDL-) induced cholestatic injury in rat sera and investigate whether they could be associated with traditional markers of liver damage, cholestasis, and fibrosis. An ELISA was performed to detect anti-VEGFR-3 autoantibodies in sera of rats with different degree of liver injury and results were correlated with aminotransferases, total bilirubin, and the relative fibrotic area. Mean absorbances of anti-VEGFR-3 autoantibodies were significantly increased from week one to week five after BDL. The highest correlation was observed with total bilirubin (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msup><mml:mrow><mml:mi>R<\/mml:mi><\/mml:mrow><mml:mrow><mml:mn fontstyle=\"italic\">2<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>= 0.8450,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">3.04<\/mml:mn><mml:mi>e<\/mml:mi><mml:mo>-<\/mml:mo><mml:mn fontstyle=\"italic\">12<\/mml:mn><\/mml:math>). In conclusion, anti-VEGFR-3 autoantibodies are early produced during BDL-induced cholestatic injury, and they are closely related to cholestasis, suggesting the potential of anti-VEGFR-3 autoantibodies as NIBMs of cholestasis in CCLDs and justifying the need for further investigations in patients with CCLD.<\/jats:p>","DOI":"10.1155\/2016\/6597970","type":"journal-article","created":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T17:02:28Z","timestamp":1461690148000},"page":"1-7","source":"Crossref","is-referenced-by-count":3,"title":["Detection of Autoantibodies to Vascular Endothelial Growth Factor Receptor-3 in Bile Duct Ligated Rats and Correlations with a Panel of Traditional Markers of Liver Diseases"],"prefix":"10.1155","volume":"2016","author":[{"given":"Florent","family":"Duval","sequence":"first","affiliation":[{"name":"Catedra de Terapia Celular, Escuela de Medicina, Tecnol\u00f3gico de Monterrey, Avenida Morones Prieto 3000 Pte., 64710 Monterrey, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delia Elva","family":"Cruz-Vega","sequence":"additional","affiliation":[{"name":"Catedra de Terapia Celular, Escuela de Medicina, Tecnol\u00f3gico de Monterrey, Avenida Morones Prieto 3000 Pte., 64710 Monterrey, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivonne","family":"Gonz\u00e1lez-Gamboa","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas (CBGP), UPM-INIA, Campus de Montegancedo, Autov\u00eda M40, Km 38, Pozuelo de Alarc\u00f3n, 28223 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"Gonz\u00e1lez-Garza","sequence":"additional","affiliation":[{"name":"Catedra de Terapia Celular, Escuela de Medicina, Tecnol\u00f3gico de Monterrey, Avenida Morones Prieto 3000 Pte., 64710 Monterrey, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Ponz","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas (CBGP), UPM-INIA, Campus de Montegancedo, Autov\u00eda M40, Km 38, Pozuelo de Alarc\u00f3n, 28223 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flora","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas (CBGP), UPM-INIA, Campus de Montegancedo, Autov\u00eda M40, Km 38, Pozuelo de Alarc\u00f3n, 28223 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Alarc\u00f3n-Galv\u00e1n","sequence":"additional","affiliation":[{"name":"Servicio de Anatom\u00eda Patol\u00f3gica y Citopatolog\u00eda, Hospital Universitario \u201cDr. Jos\u00e9 Eleuterio Gonz\u00e1lez\u201d, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Madero y Dr. Aguirre Peque\u00f1o, 64460 Monterrey, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge E.","family":"Moreno-Cuevas","sequence":"additional","affiliation":[{"name":"Catedra de Terapia Celular, Escuela de Medicina, Tecnol\u00f3gico de Monterrey, Avenida Morones Prieto 3000 Pte., 64710 Monterrey, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.04.009"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12128"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1097\/meg.0b013e32832e0a2f"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2015.05.005"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2011.02.001"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-014-0027-4"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073133"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22742"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-015-0751-2"},{"issue":"4","key":"10","first-page":"1079","volume":"27","year":"2005","journal-title":"International Journal of Oncology"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v7.i11.1581"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103259"},{"issue":"55","key":"13","first-page":"25","volume":"51","year":"2004","journal-title":"Hepato-Gastroenterology"},{"first-page":"13","volume-title":"What is an autoantibody?","year":"2014","key":"14"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2014.02.004"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.09.006"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.3350\/cmh.2013.19.2.99"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.12.034"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9440(10)65657-x"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060726"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s13317-013-0046-7"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i3.385"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2007.11.003"},{"key":"24","first-page":"125","volume":"21","year":"2007","journal-title":"The FASEB Journal"},{"issue":"1","key":"25","first-page":"71","volume":"1","year":"2012","journal-title":"Translational Gastrointestinal Cancer"},{"key":"26","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1111\/j.1478-3231.2009.01997.x","volume":"29","year":"2009","journal-title":"Liver International"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-008-0521-0"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.3978\/j.issn.2304-3881.2012.10.11"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2013.09.002"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1103545"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2010.02309.x"},{"volume":"3, article 17","journal-title":"The Pan African Medical Journal","year":"2009","key":"32"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(97)80330-3"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1136\/gut.20.2.137"},{"issue":"6","key":"35","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1016\/0016-5085(87)90618-4","volume":"92","year":"1987","journal-title":"Gastroenterology"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1172\/jci24282"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-012-0727-4"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2012.11.002"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2011.00842.x"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1136\/gut.50.4.571"}],"container-title":["Disease Markers"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2016\/6597970.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2016\/6597970.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2016\/6597970.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,24]],"date-time":"2017-06-24T07:49:59Z","timestamp":1498290599000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/dm\/2016\/6597970\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":40,"alternative-id":["6597970","6597970"],"URL":"https:\/\/doi.org\/10.1155\/2016\/6597970","relation":{},"ISSN":["0278-0240","1875-8630"],"issn-type":[{"type":"print","value":"0278-0240"},{"type":"electronic","value":"1875-8630"}],"subject":[],"published":{"date-parts":[[2016]]}}}