{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:51:09Z","timestamp":1772164269898,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>\n                    Diagnosis of the endemic cutaneous leishmaniasis (CL) in Jordan relies on patient clinical presentation and microscopic identification. Studies toward improved identification of the causative\n                    <jats:italic>Leishmania<\/jats:italic>\n                    species, especially in regions where multiple species exist, and the introduction of these techniques into medical diagnosis is paramount. This study looked at the current epidemiology of CL in Jordan. Clinically diagnosed 41 patients with CL were tested for the presence of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    parasite using both Giemsa staining from skin scraps on glass slides and ITS1-PCR from samples blotted onto storage cards (NucleoCards\u00ae). Microscopically, 28 out of the 41 (68.3%) collected samples were positive for amastigotes, whereas the molecular ITS1-PCR amplification successfully identified 30 of the 41 samples (73.2%). Furthermore, PCR-RFLP analysis allowed species identification which is impossible microscopically. Of the 30 PCR positive samples, 28 were\n                    <jats:italic>Leishmania major<\/jats:italic>\n                    positive and the other two samples were\n                    <jats:italic>Leishmania tropica<\/jats:italic>\n                    . This indicates that\n                    <jats:italic>L. major<\/jats:italic>\n                    is the most prevalent species in Jordan and the two\n                    <jats:italic>L. tropica<\/jats:italic>\n                    cases originated from Syria indicating possible future\n                    <jats:italic>L. tropica<\/jats:italic>\n                    outbreaks. Diagnosis of CL based on clinical presentation only may falsely increase its prevalence. Although PCR is more sensitive, it is still not available in our medical laboratories in Jordan.\n                  <\/jats:p>","DOI":"10.1155\/2016\/6871739","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T17:04:57Z","timestamp":1466010297000},"page":"1-7","source":"Crossref","is-referenced-by-count":15,"title":["Molecular Diagnosis and Identification of\n                    <i>Leishmania<\/i>\n                    Species in Jordan from Saved Dry Samples"],"prefix":"10.1155","volume":"2016","author":[{"given":"Nawal","family":"Hijjawi","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Hashemite University, Zarqa 13133, Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalil A.","family":"Kanani","sequence":"additional","affiliation":[{"name":"Parasitic and Zoonotic Diseases Department, Communicable Disease Directorate, Ministry of Health, Amman 11855, Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malak","family":"Rasheed","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Hashemite University, Zarqa 13133, Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manar","family":"Atoum","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Hashemite University, Zarqa 13133, Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mona","family":"Abdel-Dayem","sequence":"additional","affiliation":[{"name":"Princess Iman Center for Research and Laboratory Sciences, King Hussein Medical Center, Jordanian Royal Medical Services, Amman 11121, Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad R.","family":"Irhimeh","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Dentistry and Health 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