{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T02:28:12Z","timestamp":1793413692963,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>Staphylococci are the leading cause of nosocomial blood stream infections. Fast and accurate identification of staphylococci and confirmation of their methicillin resistance are crucial for immediate treatment with effective antibiotics. A multiplex real-time PCR assay that targets<jats:italic>mecA<\/jats:italic>,<jats:italic>femA<\/jats:italic>specific for<jats:italic>S. aureus<\/jats:italic>,<jats:italic>femA<\/jats:italic>specific for<jats:italic>S. epidermidis<\/jats:italic>,<jats:italic>16S rRNA<\/jats:italic>for universal bacteria, and<jats:italic>16S rRNA<\/jats:italic>specific for staphylococci was developed and evaluated with 290 clinical blood culture samples containing Gram-positive cocci in clusters (GPCC). For the 262 blood cultures identified to the species level with the MicroScan WalkAway system (Siemens Healthcare Diagnostics, USA), the direct real-time PCR assay of positive blood cultures showed very good agreement for the categorization of staphylococci into methicillin-resistant<jats:italic>S. aureus<\/jats:italic>(MRSA), methicillin-susceptible<jats:italic>S. aureus<\/jats:italic>(MSSA), methicillin-resistant<jats:italic>S. epidermidis<\/jats:italic>(MRSE), methicillin-susceptible<jats:italic>S. epidermidis<\/jats:italic>(MSSE), methicillin-resistant non-<jats:italic>S. epidermidis<\/jats:italic>CoNS (MRCoNS), and methicillin-susceptible non-<jats:italic>S. epidermidis<\/jats:italic>CoNS (MSCoNS) (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>\u03ba<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.9313<\/mml:mn><\/mml:math>). The direct multiplex real-time PCR assay of positive blood cultures containing GPCC can provide essential information at the critical point of infection with a turnaround time of no more than 4\u2009h. Further studies should evaluate the clinical outcome of using this rapid real-time PCR assay in glycopeptide antibiotic therapy in clinical settings.<\/jats:p>","DOI":"10.1155\/2016\/6913860","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T17:04:14Z","timestamp":1466010254000},"page":"1-7","source":"Crossref","is-referenced-by-count":15,"title":["Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal Bacteremia"],"prefix":"10.1155","volume":"2016","author":[{"given":"Yousun","family":"Chung","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taek Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Gi","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Ji","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Su","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang 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