{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:43Z","timestamp":1740169783961,"version":"3.37.3"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81360224","2015cztcxyd03"],"award-info":[{"award-number":["81360224","2015cztcxyd03"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Inner Mongolia Major Basic Science Research Program","award":["81360224","2015cztcxyd03"],"award-info":[{"award-number":["81360224","2015cztcxyd03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. To validate the ability of<jats:sup>99m<\/jats:sup>Tc-Annexin V to visualize early stage of glucocorticoid-induced femoral head necrosis by comparing with<jats:sup>99m<\/jats:sup>Tc-MDP bone scanning.<jats:italic>Methods<\/jats:italic>. Femoral head necrosis was induced in adult New Zealand white rabbits by intramuscular injection of methylprednisolone.<jats:sup>99m<\/jats:sup>Tc-Annexin scintigraphy and<jats:sup>99m<\/jats:sup>Tc-MDP scans were performed before and 5, 6, and 8 weeks after methylprednisolone administration. Rabbits were sacrificed at various time points and conducted for TUNEL and H&amp;E staining.<jats:italic>Results<\/jats:italic>. All methylprednisolone treated animals developed femoral head necrosis; at 8 weeks postinjection, destruction of bone structure was evident in H&amp;E staining, and apoptosis was confirmed by the TUNEL assay. This was matched by<jats:sup>99m<\/jats:sup>Tc-Annexin V images, which showed a significant increase in signal over baseline. Serial<jats:sup>99m<\/jats:sup>Tc-Annexin V scans revealed that increased<jats:sup>99m<\/jats:sup>Tc-Annexin V uptake could be observed in 5 weeks. In contrast, there was no effect on<jats:sup>99m<\/jats:sup>Tc-MDP signal until 8 weeks. The TUNEL assay revealed that bone cell apoptosis occurred at 5 weeks.<jats:italic>Conclusion<\/jats:italic>.<jats:sup>99m<\/jats:sup>Tc-Annexin V is superior to<jats:sup>99m<\/jats:sup>Tc-MDP for the early detection of glucocorticoid-induced femoral head necrosis in the rabbit and may be a better strategy for the early detection of glucocorticoid-induced femoral head necrosis in patients.<\/jats:p>","DOI":"10.1155\/2016\/7067259","type":"journal-article","created":{"date-parts":[[2016,2,16]],"date-time":"2016-02-16T16:04:00Z","timestamp":1455638640000},"page":"1-6","source":"Crossref","is-referenced-by-count":1,"title":["The Role of<sup>99m<\/sup>Tc-Annexin V Apoptosis Scintigraphy in Visualizing Early Stage Glucocorticoid-Induced Femoral Head Osteonecrosis in the Rabbit"],"prefix":"10.1155","volume":"2016","author":[{"given":"Xiaolong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Surgery, Affiliated Hospital of Inner Mongolia Medical University, No. 1 Tongdao North Street, Hohhot, Inner Mongolia 010050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Surgery, Affiliated Hospital of Inner Mongolia Medical University, No. 1 Tongdao North Street, Hohhot, Inner Mongolia 010050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, Affiliated Hospital of Inner Mongolia Medical University, No. 1 Tongdao North Street, Hohhot, Inner Mongolia 010050, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Surgery, Affiliated Hospital of Inner Mongolia Medical University, No. 1 Tongdao North Street, Hohhot, Inner Mongolia 010050, 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