{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T02:34:57Z","timestamp":1791513297210,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,8,7]],"date-time":"2016-08-07T00:00:00Z","timestamp":1470528000000},"content-version":"vor","delay-in-days":219,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>This study investigates morphological and biochemistry effects of binge ethanol consumption in parotid (PG) and submandibular (SG) salivary glands of rats from adolescence to adulthood. Female Wistar rats (<jats:italic>n<\/jats:italic> = 26) received ethanol at 3\u2009g\/kg\/day (20% w\/v) for 3 consecutive days\/week from the 35th until the 62nd day of life. Animals were treated in two periods: 1 week (G1) and 4 weeks (G2), with a control (treated with distilled water) and an ethanol group to each period. In morphological analysis, morphometric and immunohistochemistry evaluation for smooth muscle actin (<jats:italic>\u03b1<\/jats:italic>SMA), cytokeratin\u201018 (CK\u201018), and vimentin (VIM) were made. Biochemical changes were analyzed by concentration of nitrites and levels of malondialdehyde (MDA). The difference between groups in each analysis was evaluated by Mann\u2010Whitney <jats:italic>U<\/jats:italic> test or Student\u2019s <jats:italic>t<\/jats:italic>\u2010test (<jats:italic>p<\/jats:italic> \u2264 0.05). PG showed, at one week of ethanol exposure, lower CK\u201018 and <jats:italic>\u03b1<\/jats:italic>\u2010SMA expression, as well as MDA levels. After four weeks, lower CK\u201018 and higher MDA levels were observed in PG exposed to ethanol, in comparison to control group. SG showed lower <jats:italic>\u03b1<\/jats:italic>\u2010SMA expression after 1 and 4 weeks of ethanol exposure as well as higher MDA levels after 1 week. Ethanol binge consumption during adolescence promotes tissue and biochemical changes with only one\u2010week binge in acinar and myoepithelial PG cells.<\/jats:p>","DOI":"10.1155\/2016\/7323627","type":"journal-article","created":{"date-parts":[[2016,8,7]],"date-time":"2016-08-07T21:02:39Z","timestamp":1470603759000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats"],"prefix":"10.1155","volume":"2016","author":[{"given":"Nathalia Carolina Fernandes","family":"Fagundes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luanna Melo Pereira","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Sousa de Oliveira","family":"Paraense","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5051-7129","authenticated-orcid":false,"given":"Paulo Mecenas Alves","family":"de Farias-Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Bruno","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Melo","family":"Alves-Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o de Jesus Viana","family":"Pinheiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elena","family":"Crespo-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4493-7375","authenticated-orcid":false,"given":"Cristiane Socorro Ferraz","family":"Maia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1486-4013","authenticated-orcid":false,"given":"Rafael Rodrigues","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,7]]},"reference":[{"key":"e_1_2_7_1_2","volume-title":"Global Status Report on Alcohol and Health 2014","author":"WHO","year":"2014"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsycho.2014.01.005"},{"key":"e_1_2_7_3_2","unstructured":"II levantamento nacional de \u00e1lcool e drogas 2013 http:\/\/inpad.org.br\/lenad\/."},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/930795"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.4321\/s1135-57272014000400007"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1007\/s40211-013-0066-6","article-title":"Adolescence and Alcohol: a review of the literature","volume":"27","author":"Skala K.","year":"2013","journal-title":"Neuropsychiatrie: Klinik, Diagnostik, Therapie und Rehabilitation"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/10826084.2012.666313"},{"key":"e_1_2_7_8_2","article-title":"Alcohol consumption in late adolescence and early adulthood\u2014where is the problem?","volume":"143","author":"Kuntsche E.","year":"2013","journal-title":"Swiss Medical Weekly"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agt046"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.21192"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2008.00648.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2014.10.004"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.14670\/HH-11-604"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2004.11.013"},{"key":"e_1_2_7_15_2","volume-title":"Results from the 2013 National Survey on Drug Use and Health: National Findings","author":"Substance Abuse and Mental Health Services Administration","year":"2014"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2008.08.005"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0149-7634(00)00014-2"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1308.002"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20167"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-010-1629-7"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6451927"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)86134-h"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_7_24_2","unstructured":"National Institute on Alcohol Abuse and Alcoholism (NIAAA) Drinking Levels Defined 2016 https:\/\/www.niaaa.nih.gov\/alcohol-health\/overview-alcohol-consumption\/moderate-binge-drinking."},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnhum.2014.00405"},{"key":"e_1_2_7_26_2","first-page":"2485","article-title":"Immunohistochemical detection of cytokeratin 18 and its neo-epitope in human salivary glands and pleomorphic adenomas","volume":"20","author":"Kusama K.","year":"2000","journal-title":"Anticancer Research"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.4067\/s0716-98681998000100003"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.7860\/jcdr\/2015\/11372.5707"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(98)90038-5"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12307-010-0058-z"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1177\/0748233712457452"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1177\/153537020222700901"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-8329(00)00133-6"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(76)90140-0"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0003-9969(02)00057-2"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2016\/7323627.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2016\/7323627.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2016\/7323627","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T01:31:14Z","timestamp":1722907874000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2016\/7323627"}},"subtitle":[],"editor":[{"given":"Francisco J.","family":"Romero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1155\/2016\/7323627"],"URL":"https:\/\/doi.org\/10.1155\/2016\/7323627","archive":["Portico"],"relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1]]},"assertion":[{"value":"2016-03-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-08-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"7323627"}}