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The purpose of this study was to compare the effects of a potential immunomodulator, natural polyphenol N-feruloylserotonin (N-f-5HT), with methotrexate (MTX), the standard in RA therapy, in the chronic phase of adjuvant-induced arthritis (AA) in male Lewis rats. The experiment included healthy controls (CO), arthritic animals (AA), AA given N-f-5HT (AA-N-f-5HT), and AA given MTX (AA-MTX). N-f-5HT did not affect the body weight change and clinical parameters until the 14th experimental day. Its positive effect was rising during the 28-day experiment, indicating a delayed onset of N-f-5HT action. Administration of either N-f-5HT or MTX caused reduction of inflammation measured as the level of CRP in plasma and the activity of LOX in the liver. mRNA transcription of TNF-<jats:italic>\u03b1<\/jats:italic>and iNOS in the liver was significantly attenuated in both MTX and N-f-5HT treated groups of arthritic rats. Interestingly, in contrast to MTX, N-f-5HT significantly lowered the level of IL-1<jats:italic>\u03b2<\/jats:italic>in plasma and IL-1<jats:italic>\u03b2<\/jats:italic>mRNA expression in the liver and spleen of arthritic rats. This speaks for future investigations of N-f-5HT as an agent in the treatment of RA in combination therapy with MTX.<\/jats:p>","DOI":"10.1155\/2016\/7509653","type":"journal-article","created":{"date-parts":[[2016,7,31]],"date-time":"2016-07-31T17:02:15Z","timestamp":1469984535000},"page":"1-12","source":"Crossref","is-referenced-by-count":11,"title":["Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8732-7054","authenticated-orcid":true,"given":"\u013dudmila","family":"Pa\u0161kov\u00e1","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, 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