{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:55Z","timestamp":1740169795953,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. To investigate the effect of 2-Methoxyestradiol (2ME2) on experimental autoimmune uveitis (EAU) and the mechanism.<jats:italic>Method<\/jats:italic>. C57BL\/6 male mice were used to establish the EAU model. 2ME2 was abdominal administrated in D0\u2013D13, D0\u2013D6, and D7\u2013D13 and control group was given vehicle from D0\u2013D13. At D14, pathological severity was scored. Lymphocyte reaction was measured using MTT assay. T cell differentiation in draining lymph nodes and eye-infiltrating cells was tested by flow cytometry. Proinflammatory cytokines production from lymphocytes was determined by ELISA.<jats:italic>Result<\/jats:italic>. The disease scores from 2ME2 D0\u2013D13, 2ME2 D0\u2013D6, 2ME2 D7\u2013D13, and vehicle groups were<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn fontstyle=\"italic\">0.20<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.12<\/mml:mn><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn fontstyle=\"italic\">1.42<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.24<\/mml:mn><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mn fontstyle=\"italic\">2.25<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.32<\/mml:mn><\/mml:math>, and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mn fontstyle=\"italic\">2.42<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.24<\/mml:mn><\/mml:math>. Cells from all 2ME2 treated groups responded weaker than control (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>). The inhibitory effect of 2ME2 on lymphocyte proliferation attenuated from 2ME2 D0\u2013D13 to 2ME2 D0\u2013D6 and to 2ME2 D7\u2013D13 groups (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>). 2ME2 treated mice developed fewer Th1 and Th17 cells both in draining lymph nodes and in eyes than control (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>). Lymphocytes from 2ME2 group secreted less IFN-<jats:italic>\u03b3<\/jats:italic>and IL-17A than those from control (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M8\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>).<jats:italic>Conclusion<\/jats:italic>. 2ME2 ameliorated EAU progression and presented a better effect at priming phase. The possible mechanism could be the inhibitory impact on IRBP specific lymphocyte proliferation and Th1 and Th17 cell differentiation.<\/jats:p>","DOI":"10.1155\/2016\/7948345","type":"journal-article","created":{"date-parts":[[2016,5,8]],"date-time":"2016-05-08T21:00:54Z","timestamp":1462741254000},"page":"1-8","source":"Crossref","is-referenced-by-count":4,"title":["2-Methoxyestradiol Alleviates Experimental Autoimmune Uveitis by Inhibiting Lymphocytes Proliferation and T Cell Differentiation"],"prefix":"10.1155","volume":"2016","author":[{"given":"Linxinyu","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Shanghai Tenth People\u2019s Hospital, Tongji University School of Medicine, Shanghai 200092, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianshu","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Tenth People\u2019s Hospital, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai 200092, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaobo","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Shanghai Tenth People\u2019s Hospital, Tongji University School of Medicine, Shanghai 200092, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2332-7810","authenticated-orcid":true,"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Shanghai Tenth People\u2019s Hospital, Tongji University School of Medicine, Shanghai 200092, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"3","key":"1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.ajo.2005.03.057","volume":"140","year":"2005","journal-title":"American Journal of 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