{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:22:32Z","timestamp":1775056952488,"version":"3.50.1"},"reference-count":71,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["P30 DK56338"],"award-info":[{"award-number":["P30 DK56338"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>Sucrase insufficiency has been observed in children with of functional bowel disorders (FBD) and symptoms of dietary carbohydrate intolerance may be indistinguishable from those of FBD. A two-phase<jats:sup>13<\/jats:sup>C-sucrose\/<jats:sup>13<\/jats:sup>C-glucose breath test (<jats:sup>13<\/jats:sup>C-S\/GBT) was used to assess sucrase activity because disaccharidase assays are seldom performed in adults. When<jats:sup>13<\/jats:sup>C-sucrose is hydrolyzed to liberate monosaccharides, oxidation to<jats:sup>13<\/jats:sup>CO<jats:sub>2<\/jats:sub>is a proportional indicator of sucrase activity. Subsequently,<jats:sup>13<\/jats:sup>C-glucose oxidation rate was determined after a secondary substrate ingestion (superdose) to adjust for individual habitus effects (Phase II).<jats:sup>13<\/jats:sup>CO<jats:sub>2<\/jats:sub>enrichment recovery ratio from<jats:sup>13<\/jats:sup>C-sucrose and secondary<jats:sup>13<\/jats:sup>C-glucose loads reflect the individualized sucrase activity [<jats:italic>Coefficient of Glucose Oxidation for Sucrose<\/jats:italic>(CGO-S)]. To determine if sucrase insufficiency could be a factor in FBD,<jats:sup>13<\/jats:sup>C-S\/GBT was validated using subjects with known sucrase gene mutation status by comparing<jats:sup>13<\/jats:sup>CO<jats:sub>2<\/jats:sub>-breath enrichment with plasma<jats:sup>13<\/jats:sup>C-glucose enrichment.<jats:sup>13<\/jats:sup>C-S\/GBT was used to assess sucrose digestion in FBD patients and asymptomatic controls.<jats:sup>13<\/jats:sup>CO<jats:sub>2<\/jats:sub>-breath enrichment correlated with the appearance of<jats:sup>13<\/jats:sup>C-sucrose-derived glucose in plasma (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:msup><mml:mrow><mml:mi>r<\/mml:mi><\/mml:mrow><mml:mrow><mml:mn fontstyle=\"italic\">2<\/mml:mn><\/mml:mrow><\/mml:msup><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.80<\/mml:mn><\/mml:math>). Mean, control group CGO-S-enrichment outcomes were 1.01 at 60\u2032, 0.92 at 75\u2032, and 0.96 at mean 60\u2032\u201375\u2032 with normal CGO-S defined as &gt;0.85 (95% C.I.). In contrast, FBD patients demonstrated lower CGO-S values of 0.77 at 60\u2032, 0.77 at 75\u2032, and 0.76 at mean 60\u2032\u201375\u2032 (<jats:italic>Chi Square: 6.55<\/jats:italic>;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><\/mml:math>), which points to sucrose maldigestion as a cause of FBD.<\/jats:p>","DOI":"10.1155\/2016\/7952891","type":"journal-article","created":{"date-parts":[[2016,8,8]],"date-time":"2016-08-08T17:01:58Z","timestamp":1470675718000},"page":"1-12","source":"Crossref","is-referenced-by-count":15,"title":["Use of the Biphasic<sup>13<\/sup>C-Sucrose\/Glucose Breath Test to Assess Sucrose Maldigestion in Adults with Functional Bowel Disorders"],"prefix":"10.1155","volume":"2016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4837-6480","authenticated-orcid":true,"given":"Antone R.","family":"Opekun","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Division of Gastroenterology, Nutrition and Hepatology, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert M.","family":"Balesh","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold T.","family":"Shelby","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.0954"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2013.15"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-015-1076-x"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.11.061"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2009.07.005"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.02.037"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2014.127"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.34755"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2010.47"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.27995"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1586\/17474124.2015.1067138"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1056\/nejmra1207068"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2011.201"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2013.09.046"},{"issue":"4","key":"15","first-page":"S-654","volume":"148","year":"2015","journal-title":"Gastroenterology"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2005.00745.x"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1177\/0148607115594675"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1097\/SMJ.0b013e3181b64c7f"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jada.2006.07.010"},{"key":"20","first-page":"111","volume":"216","year":"1996","journal-title":"Scandinavian Journal of Gastroenterology. 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