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Au-based coatings are deposited onto a nonadiabatic tapered optical fibre using (i) a novel facile method composed of layer-by-layer deposition consisting of polycation (poly(allylamine hydrochloride), PAH) and negatively charged SiO<jats:sub>2<\/jats:sub>nanoparticles (NPs) followed by the deposition of the charged Au NPs and (ii) the sputtering technique. The Au NPs and Au thin film surfaces are then modified with biotin in order to bind streptavidin (SV) molecules and detect them. The sensing principle is based on the sensitivity of the transmission spectrum of the device to changes in the refractive index of the coatings induced by the SV binding to the biotin. Both sensors showed high sensitivity to SV, with the lowest measured concentration levels below 2.5\u2009nM. The calculated binding constant for the biotin-SV pair was<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn fontstyle=\"italic\">2.2<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn fontstyle=\"italic\">10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mo>-<\/mml:mo><mml:mn fontstyle=\"italic\">11<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>\u2009M<jats:sup>\u22121<\/jats:sup>when a tapered fibre modified with the LbL method was used, with a limit of detection (LoD) of 271\u2009pM. The sensor formed using sputtering had a binding constant of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn fontstyle=\"italic\">1.01<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn fontstyle=\"italic\">10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mo>-<\/mml:mo><mml:mn fontstyle=\"italic\">10<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>\u2009M<jats:sup>\u22121<\/jats:sup>with a LoD of 806\u2009pM. These new structures and their simple fabrication technique could be used to develop other biosensors.<\/jats:p>","DOI":"10.1155\/2016\/8129387","type":"journal-article","created":{"date-parts":[[2016,8,19]],"date-time":"2016-08-19T02:08:42Z","timestamp":1471572522000},"page":"1-11","source":"Crossref","is-referenced-by-count":14,"title":["Novel Highly Sensitive Protein Sensors Based on Tapered Optical Fibres Modified with Au-Based Nanocoatings"],"prefix":"10.1155","volume":"2016","author":[{"given":"Aitor","family":"Urrutia","sequence":"first","affiliation":[{"name":"Nanostructured Optical Devices Laboratory, Electric and Electronic Engineering Department, Public University of Navarra, Campus Arrosadia, 31006 Pamplona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kartheka","family":"Bojan","sequence":"additional","affiliation":[{"name":"Advanced Optics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham NG7 2RD, 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