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After irradiation, AT cells showed checkpoint impairment and a fraction of cells displayed an abnormal centrosome number and tetraploid DNA content, and this fraction increased along with apoptosis rates. At all times analyzed, AT cells displayed a significantly higher rate of radiation-induced apoptosis than normal cells. Besides apoptosis, 70\u201385% of the AT viable cells (TUNEL-negative) carried \u226510<jats:italic>\u03b3<\/jats:italic>H2AX foci\/cell, while only 12\u201327% of normal cells did. The fraction of AT and normal cells undergoing early and late apoptosis were isolated by flow cytometry and residual DSBs were concretely scored in these populations. Half of the<jats:italic>\u03b3<\/jats:italic>H2AX-positive AT cells undergoing early apoptosis carried \u226510<jats:italic>\u03b3<\/jats:italic>H2AX foci\/cell and this fraction increased to 75% in late apoptosis. The results suggest that retention of DNA damage-induced<jats:italic>\u03b3<\/jats:italic>H2AX foci is an indicative of lethal DNA damage, as cells undergoing apoptosis are those accumulating more DSBs. Scoring of residual<jats:italic>\u03b3<\/jats:italic>H2AX foci might function as a predictive tool to assess radiation-induced apoptosis.<\/jats:p>","DOI":"10.1155\/2016\/8279560","type":"journal-article","created":{"date-parts":[[2016,1,6]],"date-time":"2016-01-06T21:05:33Z","timestamp":1452114333000},"page":"1-12","source":"Crossref","is-referenced-by-count":11,"title":["Analysis of Residual DSBs in Ataxia-Telangiectasia Lymphoblast Cells Initiating Apoptosis"],"prefix":"10.1155","volume":"2016","author":[{"given":"Teresa","family":"Anglada","sequence":"first","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, Edifici C, Bellaterra, 08193 Cerdanyola del Vall\u00e8s, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariona","family":"Terradas","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de 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