{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T23:53:32Z","timestamp":1769644412727,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30800812"],"award-info":[{"award-number":["30800812"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Background.<\/jats:italic>Chickens serve as an intermediate host for<jats:italic>Toxoplasma gondii<\/jats:italic>and<jats:italic>Neospora caninum<\/jats:italic>; infection of free-range (FR) chickens with these organisms is a useful indicator of soil and environmental contamination with oocysts. A total of 700 FR chicken serum samples and 300 heart samples were collected from Henan province from March to July 2015. Antibodies to<jats:italic>T. gondii<\/jats:italic>were found in 18.86% (132\/700) of the chickens by modified agglutination test (cut-off 1\u2009:\u200925), while 23.14% (162\/700) were positive for<jats:italic>N. caninum<\/jats:italic>by indirect fluorescent antibody test (cut-off 1\u2009:\u200925).<jats:italic>T. gondii<\/jats:italic>DNA was detected in the myocardium digestion liquids of 4\/25 (16%) FR chickens. The PCR results of<jats:italic>N. caninum<\/jats:italic>DNA from FR chicken myocardium digestion liquids (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">25<\/mml:mn><\/mml:math>) were all negative. Attempts to isolate viable<jats:italic>T. gondii<\/jats:italic>were unsuccessful. The results showed that there were antibodies to<jats:italic>T. gondii<\/jats:italic>and<jats:italic>N. caninum<\/jats:italic>in FR chickens from Henan province. Accordingly, effective control of feces from cats and dogs and improved pets hygiene habits were needed. To the author\u2019s knowledge, this is the first report of the presence of<jats:italic>N. caninum<\/jats:italic>antibody in chickens from China.<\/jats:p>","DOI":"10.1155\/2016\/8290536","type":"journal-article","created":{"date-parts":[[2016,5,5]],"date-time":"2016-05-05T17:02:06Z","timestamp":1462467726000},"page":"1-5","source":"Crossref","is-referenced-by-count":16,"title":["<i>Toxoplasma gondii<\/i>and<i>Neospora caninum<\/i>in Free-Range Chickens in Henan Province of China"],"prefix":"10.1155","volume":"2016","author":[{"given":"Yongjie","family":"Feng","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoyao","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, 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