{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T22:44:20Z","timestamp":1789080260219,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>VEGF and podoplanin (PDPN) have been identified as angiogenesis and\/or lymphangiogenesis regulators and might be essential to restrict tumor growth, progression, and metastasis. In the present study, we evaluate the association between the expression of these markers and CIN grade. Immunohistochemistry was performed in 234 uterine cervical samples using conventional histologic sections or TMA with the monoclonal antibodies to VEGF (C-1 clone) and podoplanin (D2-40 clone). Positive-staining rates of VEGF in 191 CIN specimens were significantly associated with histological grade (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>). Negative and\/or focal immunostaining for PDPN were more frequent in CIN 3 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.016<\/mml:mn><\/mml:math>). We found that patients with CIN 3 more frequently had strong and more diffuse staining for VEGF and diminished staining for PDPN (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.018<\/mml:mn><\/mml:math>). Strong and more diffuse VEGF immunoexpressions in CIN 2 and CIN 3 were detected when compared to CIN 1. Negative and\/or focal PDPN immunoexpression appear to be more frequent in CIN 3. Moderate to strong VEGF expression may be a tendency among patients with high-grade lesions and diminished PDPN expression.<\/jats:p>","DOI":"10.1155\/2016\/8293196","type":"journal-article","created":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T21:34:59Z","timestamp":1464125699000},"page":"1-8","source":"Crossref","is-referenced-by-count":8,"title":["Immunohistochemical Expression of VEGF and Podoplanin in Uterine Cervical Squamous Intraepithelial Lesions"],"prefix":"10.1155","volume":"2016","author":[{"given":"Patr\u00edcia Napoli","family":"Belfort-Mattos","sequence":"first","affiliation":[{"name":"Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of S\u00e3o Paulo, UNIFESP\/EPM, 380 Borges Lagoa Street, 04038-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Rubino de Azevedo","family":"Focchi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Federal University of S\u00e3o Paulo, UNIFESP\/EPM, 740 Botucatu Street, 04023-062 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julisa Chamorro Lascasas","family":"Ribalta","sequence":"additional","affiliation":[{"name":"Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of S\u00e3o Paulo, UNIFESP\/EPM, 380 Borges Lagoa Street, 04038-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Megale De Lima","sequence":"additional","affiliation":[{"name":"Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of S\u00e3o Paulo, UNIFESP\/EPM, 380 Borges Lagoa Street, 04038-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen Regina","family":"Nogueira Carvalho","sequence":"additional","affiliation":[{"name":"Gynecological Disease Prevention Nucleus (NUPREV), 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