{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:03:09Z","timestamp":1773799389450,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>MicroRNAs (miRNAs) play critical roles in the progression of laryngeal cancer (LC). In this study, we aimed to investigate whether miR-149 is associated with the prognosis of patients with LC. A total of 97 laryngeal squamous cell carcinoma patients who underwent tumor resection were included in our follow-up study.<jats:italic>In vitro<\/jats:italic>studies was performed in cancer cell line Hep-2 to explore the antitumor role of miR-149 in LC. We found that the expression of miR-149 was significantly lower in tumor tissues, compared with vocal cord polyp tissues (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>). Kaplan-Meier analysis revealed that miR-149 expression status is significantly associated with survival duration (log rank test,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.0<\/mml:mn><mml:mn fontstyle=\"italic\">5<\/mml:mn><\/mml:math>), and multivariate Cox regression analysis revealed that patients with low miR-149 expression had shorter survival times compared with patients with high miR-149 expression.<jats:italic>In vitro<\/jats:italic>studies revealed that the exogenous expression of miRNA-149 inhibits the proliferation of human Hep-2 cells and induces cell apoptosis. Our study suggests that miR-149 expression in laryngeal squamous cell carcinoma tissues is critically associated with the prognosis of patients, and the ectopic expression of miR-149 in Hep-2 cells inhibits proliferation and cell cycle progression.<\/jats:p>","DOI":"10.1155\/2016\/8561251","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T17:04:46Z","timestamp":1466010286000},"page":"1-6","source":"Crossref","is-referenced-by-count":19,"title":["Clinical Significance of miR-149 in the Survival of Patients with Laryngeal Squamous Cell Carcinoma"],"prefix":"10.1155","volume":"2016","author":[{"given":"Yi","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Tianjin Medical University General Hospital, Tianjin 300052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Peng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Tianjin Medical University 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