{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:30:04Z","timestamp":1761611404112,"version":"3.37.3"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Analytical Chemistry"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16\/70 column packed with sepharose CL-4B with six different batches of Ingovax\u00ae ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd., Bangladesh. A quantitative rocket immunoelectrophoresis assay was employed to determine the polysaccharide contents of each serogroup. The calculated distribution coefficient values of serogroups A, C, W, and Y were found to be<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn fontstyle=\"italic\">0.26<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.16<\/mml:mn><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn fontstyle=\"italic\">0.21<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.11<\/mml:mn><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mn fontstyle=\"italic\">0.21<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.11<\/mml:mn><\/mml:math>, and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mn fontstyle=\"italic\">0.14<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn fontstyle=\"italic\">0.12<\/mml:mn><\/mml:math>, respectively, and met the requirements of British Pharmacopeia. The method was proved to be robust for determining the distribution coefficient values which is an obligatory requirement for vaccine lot release.<\/jats:p>","DOI":"10.1155\/2016\/9404068","type":"journal-article","created":{"date-parts":[[2016,9,5]],"date-time":"2016-09-05T21:02:28Z","timestamp":1473109348000},"page":"1-7","source":"Crossref","is-referenced-by-count":4,"title":["A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine"],"prefix":"10.1155","volume":"2016","author":[{"given":"Imran","family":"Khan","sequence":"first","affiliation":[{"name":"Incepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. M. Taufiqur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Incepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. M. 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