{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T16:23:09Z","timestamp":1782231789845,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. Vernal keratoconjunctivitis (VKC) is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy. Several inflammatory and tissue remodeling factors contribute to VKC disease. The aim is to provide a chip-based protein analysis in tears from patients suffering from quiescent or active VKC.<jats:italic>Methods<\/jats:italic>. This study cohort included 16 consecutive patients with VKC and 10 controls. Participants were subjected to clinical assessment of ocular surface and tear sampling. Total protein quantification, total protein sketch, and protein array (sixty protein candidates) were evaluated.<jats:italic>Results<\/jats:italic>. An overall increased Fluorescent Intensity expression was observed in VKC arrays. Particularly, IL1<jats:italic>\u03b2<\/jats:italic>, IL15, IL21, Eotaxin2, TACE, MIP1<jats:italic>\u03b1<\/jats:italic>, MIP3<jats:italic>\u03b1<\/jats:italic>, NCAM1, ICAM2,<jats:italic>\u03b2<\/jats:italic>NGF, NT4, BDNF,<jats:italic>\u03b2<\/jats:italic>FGF, SCF, MMP1, and MMP2 were increased in quiescent VKC. Of those candidates, only IL1<jats:italic>\u03b2<\/jats:italic>, IL15, IL21,<jats:italic>\u03b2<\/jats:italic>NGF, SCF, MMP2, Eotaxin2, TACE, MIP1<jats:italic>\u03b1<\/jats:italic>, MIP3<jats:italic>\u03b1<\/jats:italic>, NCAM1, and ICAM2 were increased in both active and quiescent VKC. Finally, NT4,<jats:italic>\u03b2<\/jats:italic>FGF, and MMP1 were highly increased in active VKC.<jats:italic>Conclusion<\/jats:italic>. A distinct \u201cprotein tear-print\u201d characterizes VKC activity, confirming some previously reported factors and highlighting some new candidates common to quiescent and active states. Those candidates expressed in quiescent VKC might be considered as predictive indicators of VKC reactivation and\/or exacerbation out-of-season.<\/jats:p>","DOI":"10.1155\/2016\/9672082","type":"journal-article","created":{"date-parts":[[2016,2,17]],"date-time":"2016-02-17T16:02:49Z","timestamp":1455724969000},"page":"1-10","source":"Crossref","is-referenced-by-count":20,"title":["Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation"],"prefix":"10.1155","volume":"2016","author":[{"given":"Alessandra","family":"Micera","sequence":"first","affiliation":[{"name":"IRCCS-G.B. Bietti Foundation, 00100 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Di Zazzo","sequence":"additional","affiliation":[{"name":"IRCCS-G.B. Bietti Foundation, 00100 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graziana","family":"Esposito","sequence":"additional","affiliation":[{"name":"IRCCS-G.B. Bietti Foundation, 00100 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Sgrulletta","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Campus Bio-Medico University, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia L.","family":"Calder","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology, London EC1V 9EL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Bonini","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Campus Bio-Medico University, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/s0161-6420(00)00092-0"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1097\/00130832-200310000-00011"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2012.11.018"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3282efb726"},{"issue":"1","key":"5","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1046\/j.1365-2222.2000.00699.x","volume":"30","year":"2000","journal-title":"Clinical & Experimental Allergy"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2006.02499.x"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-1759(01)00407-0"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.81.12.1099"},{"issue":"10","key":"9","first-page":"2161","volume":"38","year":"1997","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"10","first-page":"1562","volume":"13","year":"2007","journal-title":"Molecular Vision"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2005.04.009"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-006-0285-7"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.124.4.462"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.07.004"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01858.x"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.07.019"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/0039-6257(86)90119-0"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2007.122044"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1080\/09286580802573177"},{"key":"20","first-page":"2717","volume":"18","year":"2012","journal-title":"Molecular Vision"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-005-0127-z"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1021\/pr0501970"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-4-4-210"},{"issue":"2","key":"24","doi-asserted-by":"crossref","first-page":"173","DOI":"10.2144\/04372TE01","volume":"37","year":"2004","journal-title":"BioTechniques"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1378\/chest.08-0962"},{"key":"26","first-page":"97","volume":"68","year":"2013","journal-title":"Allergy"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1097\/ico.0b013e31828ffdf8"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.02.004"},{"issue":"9","key":"29","first-page":"1242","volume":"17","year":"2013","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.423"},{"issue":"1","key":"31","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1177\/039463201102400103","volume":"24","year":"2011","journal-title":"International Journal of Immunopathology and Pharmacology"},{"issue":"7","key":"32","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1177\/112067210301300702","volume":"13","year":"2003","journal-title":"European Journal of Ophthalmology"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-05-157818"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/s0161-6420(02)01767-0"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2007.0149"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/s1357-2725(01)00097-8"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1002\/jso.23247"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.2174\/187152810792231913"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2004.09.003"},{"issue":"10","key":"39","first-page":"2127","volume":"36","year":"1995","journal-title":"Investigative Ophthalmology and Visual Science"},{"issue":"13","key":"40","first-page":"4175","volume":"41","year":"2000","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1007\/s10384-005-0319-4"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0906595"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0766"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.2174\/157340606778250261"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2016\/9672082.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2016\/9672082.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2016\/9672082.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T14:08:47Z","timestamp":1567606127000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2016\/9672082\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":45,"alternative-id":["9672082","9672082"],"URL":"https:\/\/doi.org\/10.1155\/2016\/9672082","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}