{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:41:39Z","timestamp":1761597699227},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>Specific blocking strategies of TLR2-mediated inflammatory signaling and hypersensitivity reactions may offer novel therapeutic strategies to prevent a variety of diseases. In this study, we investigated the blocking effects of a new anti-TLR2 antibody anti-T20 against a 20 mer peptide T20 located in the extracellular specific domain of mouse TLR2. In addition, the effects of the anti-T20<jats:italic>in vitro<\/jats:italic>, measuring the inhibition of the IL-6 and TNF-<jats:italic>\u03b1<\/jats:italic>production in response to PGN, LTA, and Pam3CSK4-stimulated RAW264.7 cells, were determined.<jats:italic>In vivo<\/jats:italic>, the effects of anti-T20 on a lethal anaphylaxis model using PGN-challenged OVA allergic mice, including the rectal temperature and mortality, and serum levels of TNF-<jats:italic>\u03b1<\/jats:italic>, IL-6, and LTC4 were assayed. The results showed that anti-T20 specifically bound to TLR2 and significantly inhibited PGN, LTA, and Pam3CSK4-driven TNF-<jats:italic>\u03b1<\/jats:italic>and IL-6 production by RAW264.7 cells. Also, anti-T20 protected OVA allergic mice from PGN-induced lethal anaphylaxis, and the serum levels of TNF-<jats:italic>\u03b1<\/jats:italic>, IL-6, and LTC4 of anti-T20 treated PGN-challenged OVA allergic mice were decreased as compared to isotype control of anti-T20 treated mice. In summary, this study produced a new antibody against the specific extracellular domain of TLR2 which has protective effect on TLR2 agonists-driven inflammatory and allergic response.<\/jats:p>","DOI":"10.1155\/2016\/9803846","type":"journal-article","created":{"date-parts":[[2016,4,24]],"date-time":"2016-04-24T21:00:53Z","timestamp":1461531653000},"page":"1-8","source":"Crossref","is-referenced-by-count":1,"title":["Protective Effect of an Antibody against Specific Extracellular Domain of TLR2 on Agonists-Driven Inflammatory and Allergic Response"],"prefix":"10.1155","volume":"2016","author":[{"given":"Tianwu","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Burns and Plastics, The People\u2019s Hospital of Anyang City, Anyang, Henan 455000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Cai","sequence":"additional","affiliation":[{"name":"The Institute of Nephrology, Affiliated Hospital of Guangdong Medical College, 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