{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:50Z","timestamp":1740169790425,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Shandong Province Outstanding Young Scientist","award":["BS2010SW008"],"award-info":[{"award-number":["BS2010SW008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p><jats:italic>Purpose.<\/jats:italic>Using rat conjunctival bleb model, we correlated changes morphology and histology in the bleb with changes in MMP-2 and TIMP-2 levels.<jats:italic>Methods.<\/jats:italic>Filtering surgeries were performed on rats. Dynamic changes in morphology and histopathology were observed using HE staining. Expression of MMP-2 and TIMP-2 was determined by immunofluorescence microscopy and western blotting.<jats:italic>Results<\/jats:italic>. Well-elevated filtering blebs formed and persisted for an average of 12 days. Histological examination showed that inflammatory was dominant in postoperative days 1\u20133, and proliferating manifestation became the main sign 5 days later. Western blot showed that MMP-2 was downregulated 1 day after surgery, upregulated at 3 days, and observed with a peak at 7 days; then it persisted until 28 days. The difference was statistically significant (<jats:italic>F<\/jats:italic>= 280.18,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><\/mml:math>).TIMP-2 was upregulated 1 day after surgery and observed with a peak at 5 days; then it persisted until 28 days. The difference was statistically significant (<jats:italic>F<\/jats:italic>= 145.34,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><\/mml:math>).<jats:italic>Conclusions.<\/jats:italic>During the processes of conjunctival filtering bleb and scar formation in rats, the changes in MMP-2 and TIMP-2 levels in the filtering area, together with a corresponding proliferation of fibroblasts and the accumulation of collagen fibres, resulted in scarring of filtering blebs.<\/jats:p>","DOI":"10.1155\/2017\/1054129","type":"journal-article","created":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T17:00:38Z","timestamp":1505754038000},"page":"1-10","source":"Crossref","is-referenced-by-count":2,"title":["Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1736-7880","authenticated-orcid":true,"given":"Ling","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da-Bo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7676-3181","authenticated-orcid":true,"given":"Meng-Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ru-Yong","family":"Yao","sequence":"additional","affiliation":[{"name":"Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2005.081224"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1996.63"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1097\/00061198-200106000-00017"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)77017-5"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s00347-002-0698-6"},{"issue":"5","key":"6","first-page":"350","volume":"20","year":"1989","journal-title":"Ophthalmic Surgery"},{"first-page":"865","volume-title":"Wound repair: an overview","year":"1999","key":"7"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800840206"},{"issue":"8","key":"9","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1096\/fasebj.5.8.1850705","volume":"5","year":"1991","journal-title":"The FASEB Journal"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-2063"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1159\/000109987"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1159\/000067044"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1159\/000076891"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2005.03.001"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1097\/01.ijg.0000131482.86547.5a"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(52)90254-7"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.3109\/02713689609000767"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/s004170050107"},{"key":"19","first-page":"980","volume":"42","year":"2006","journal-title":"Chinese Journal of Ophthalmology"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1986.01050240109050"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-3147"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-011-1658-0"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-10120"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2015.1023460"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1097\/00004630-200021020-00010"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.2923"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2010.05.002"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06698.x"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/1054129.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/1054129.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/1054129.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T12:47:54Z","timestamp":1570106874000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2017\/1054129\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":28,"alternative-id":["1054129","1054129"],"URL":"https:\/\/doi.org\/10.1155\/2017\/1054129","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2017]]}}}