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In this study, rAAV\u2010PR39\u2010ADM, coexpressing antimicrobial peptide (PR39) and adrenomedullin (ADM), was designed with the mediation of recombinant adeno\u2010associated virus. In vitro, CRL\u20101730 cells were divided into four groups, namely, the sham group, the AAV\u2010null group, the NS (normal saline) group, and the PR39\u2010ADM group. Immunocytochemistry analysis, CCK\u20108 assays, Matrigel assays, and apoptotic analysis were performed; in vivo, myocardial infarction model was established through ligation of the left coronary artery on rats, and treatment groups corresponded to those used in vitro. Myocardial injury, cardiac performance, and the extent of myocardial apoptosis were assessed. Results suggested that rAAV\u2010PR39\u2010ADM administration after myocardial infarction improved cell viability and cardiac function, attenuated apoptosis and myocardial injury, and promoted angiogenesis. Subsequently, levels of 6\u00d7His, HIF\u20101<jats:italic>\u03b1<\/jats:italic>, VEGF, p\u2010Akt, Akt, ADM, Bcl\u20102, and Bax were measured by western blot. rAAV\u2010PR39\u2010ADM increased p\u2010Akt, HIF\u20101<jats:italic>\u03b1<\/jats:italic>, and VEGF levels and induced higher Bcl\u20102 expression and lower Bax expression. In conclusion, our results demonstrate that rAAV\u2010PR39\u2010ADM mitigates myocardial injury by promoting angiogenesis and reducing apoptosis. This study suggests a potential novel gene therapy\u2010based method that could be used clinically for myocardial infarction.<\/jats:p>","DOI":"10.1155\/2017\/1271670","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T21:01:51Z","timestamp":1488488511000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Intramyocardial Injection of Recombinant Adeno\u2010Associated Viral Vector Coexpressing PR39\/Adrenomedullin Enhances Angiogenesis and Reduces Apoptosis in a Rat Myocardial Infarction 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