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Here, we assess the involvement of stretch\u2010sensitive calcium channels and calcium mobilization in isolated livers, after chemical modifications of the endothelial glycocalyx and changing perfusion directionality. Inhibiting in vivo protein synthesis, we found that liver enzyme release is influenced by de novo synthesis of endothelial glycocalyx components, and released enzymes are confined into a liver \u201cpool.\u201d Moreover, liver enzyme release depended on extracellular calcium entry possibly mediated by stretch\u2010sensitive calcium channels, and this endothelial\u2010mediated mechanotransduction in liver enzyme release was also evidenced by modifying the glycocalyx carbohydrate components, directionality of perfusing flow rate, and the participation of nitric oxide (NO) and malondialdehyde (MDA), leading to modifications in the intracellular distribution of these enzymes mainly as nuclear enrichment of \u201cmitochondrial\u201d enzymes. In conclusion, the flow\u2010induced shear stress may provide fine\u2010tuned control of released hepatic enzymes through mediation by the endothelium glycocalyx, which provides evidence of a biological role of the enzyme release rather to be merely a biomarker for evaluating hepatotoxicity and liver damage, actually positively influencing progression of liver regeneration in mammals.<\/jats:p>","DOI":"10.1155\/2017\/1360565","type":"journal-article","created":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:19:08Z","timestamp":1488327548000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Rat Liver Enzyme Release Depends on Blood Flow\u2010Bearing Physical Forces Acting in Endothelium Glycocalyx rather than on Liver Damage"],"prefix":"10.1155","volume":"2017","author":[{"given":"Julieta A.","family":"D\u00edaz-Ju\u00e1rez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5010-775X","authenticated-orcid":false,"given":"Rolando","family":"Hern\u00e1ndez-Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,28]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/23.12.2219"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/46.12.2027"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/41.12.1882"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/lt.23634"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2013.08.015"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20081"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1590\/0004-2730000002831"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2014.07.013"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0000000000000254"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365520510024142"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1023\/a:1026601814082"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1995.75.3.519"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1332808100"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00622.2006"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.6.040803.140040"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03896"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607224103"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200709910"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710520105"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.01.137"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/lt.22238"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(95)90077-2"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.1.11"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20080163"},{"key":"e_1_2_9_25_2","first-page":"186","article-title":"Experimental pathology of the liver. 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