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There is a research need to discover novel compounds that safely and effectively regulate pigmentation. To identify novel modulators of pigmentation, we attempted to purify compounds from a bioactive fraction of the Korean medicinal plant <jats:italic>Artemisia capillaris<\/jats:italic> Thunberg. The novel compound isofraxidin 7\u2010<jats:italic>O<\/jats:italic>\u2010(6\u2032\u2010<jats:italic>O<\/jats:italic>\u2010<jats:italic>p<\/jats:italic>\u2010coumaroyl)\u2010<jats:italic>\u03b2<\/jats:italic>\u2010glucopyranoside (compound <jats:bold>1<\/jats:bold>) was isolated and its pigmentation activity was characterized in mammalian melanocytes. Compound <jats:bold>1<\/jats:bold> stimulated melanin accumulation and increased tyrosinase activity, which regulates melanin synthesis. Moreover, compound <jats:bold>1<\/jats:bold> increased the expression of tyrosinase and the key melanogenesis regulator microphthalmia\u2010associated transcription factor (MITF) in melanocytes. Compared to the parent compound, isofraxidin, compound <jats:bold>1<\/jats:bold> produced greater effects on these pigmentation parameters. To validate compound <jats:bold>1<\/jats:bold> as a novel hyperpigmentation agent in vivo, we utilized the zebrafish vertebrate model. Zebrafish treated with compound <jats:bold>1<\/jats:bold> showed higher melanogenesis and increased tyrosinase activity. Compound <jats:bold>1<\/jats:bold> treated embryos had no developmental defects and displayed normal cardiac function, indicating that this compound enhanced pigmentation without producing toxicity. In summary, our results describe the characterization of novel natural product compound <jats:bold>1<\/jats:bold> and its bioactivity as a pigmentation enhancer, demonstrating its potential as a therapeutic to treat hypopigmentation disorders.<\/jats:p>","DOI":"10.1155\/2017\/1401279","type":"journal-article","created":{"date-parts":[[2017,5,24]],"date-time":"2017-05-24T21:01:39Z","timestamp":1495659699000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Isolation and Characterization of Isofraxidin 7\u2010<i>O<\/i>\u2010(6\u2032\u2010<i>O<\/i>\u2010<i>p<\/i>\u2010Coumaroyl)\u2010<i>\u03b2<\/i>\u2010glucopyranoside from <i>Artemisia capillaris<\/i> Thunberg: A Novel, Nontoxic Hyperpigmentation Agent That Is Effective In Vivo"],"prefix":"10.1155","volume":"2017","author":[{"given":"Soon-Ho","family":"Yim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Tabassum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woong-Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haaglim","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hyung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galzad J.","family":"Batkhuu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Jung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0761-3064","authenticated-orcid":false,"given":"Won Keun","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6005-0833","authenticated-orcid":false,"given":"Da-Woon","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2555-3069","authenticated-orcid":false,"given":"Darren R.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,24]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.200900194"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(14)60763-7"},{"key":"e_1_2_9_3_2","volume-title":"Oculocutaneous Albinism Type 1","author":"Lewis RA.","year":"1993"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1011-1344(01)00212-3"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/3.1.53"},{"key":"e_1_2_9_6_2","first-page":"5740","article-title":"Differential down-regulation of epidermal protein kinase C by 12\u2013O-tetradecanoylphorbol-13-acetate and diacylglycerol: association with epidermal hyperplasia and tumor promotion","volume":"50","author":"Hansen L. 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