{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T20:32:38Z","timestamp":1787949158346,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,3]],"date-time":"2017-12-03T00:00:00Z","timestamp":1512259200000},"content-version":"vor","delay-in-days":336,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ011578"],"award-info":[{"award-number":["PJ011578"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Once breast cancer cells grow aggressively and become lodged in the skeleton through migration and invasion, they interact with bone microenvironment and accelerate much more tumor growth and bone destruction. We investigated whether liensinine and nuciferine, major active components in <jats:italic>Nelumbo nucifera<\/jats:italic> (lotus), could prevent breast cancer cell\u2010mediated bone destruction. Liensinine and nuciferine inhibited the growth of MDA\u2010MB\u2010231 and MCF\u20107 human breast cancer cells by inducing apoptosis and inhibiting proliferation via cell cycle arrest. Liensinine treatment led to the increased Bax\/Bcl\u20102 ratio, activation of caspase\u20103, and subsequent cleavage of PARP. Liensinine also displayed significant inhibition on the migration and invasion of both MDA\u2010MB\u2010231 and MCF\u20107 human breast cancer cells compared with nuciferine. In addition, liensinine and nuciferine inhibited the receptor activator of nuclear factor kappa\u2010B ligand\u2010 (RANKL\u2010) induced osteoclast differentiation in mouse bone marrow macrophage cells and mature osteoclast\u2010mediated bone resorption. Furthermore, oral administration of liensinine reduced the osteolysis in nude mice with intratibial injection of MDA\u2010MB\u2010231 cells. Collectively, liensinine and nuciferine may be promising candidates for preventing and treating breast cancer bone metastasis and the resulting osteolytic bone loss by targeting both cancer cells and osteoclasts. Liensinine has more potent anticancer and antibone resorptive activities than nuciferine.<\/jats:p>","DOI":"10.1155\/2017\/1583185","type":"journal-article","created":{"date-parts":[[2017,12,3]],"date-time":"2017-12-03T23:30:36Z","timestamp":1512343836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Liensinine and Nuciferine, Bioactive Components of <i>Nelumbo nucifera<\/i>, Inhibit the Growth of Breast Cancer Cells and Breast Cancer\u2010Associated Bone Loss"],"prefix":"10.1155","volume":"2017","author":[{"given":"Eun Ji","family":"Kang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Kyoung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Kyun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Hwa","family":"Son","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki Rim","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8428-9005","authenticated-orcid":false,"given":"Won-Yoon","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,3]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.2217\/fon.09.138"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncponc1323"},{"key":"e_1_2_7_4_2","first-page":"49","article-title":"Molecular biology of bone remodelling","volume":"5","author":"Rucci N.","year":"2008","journal-title":"Clinical Cases in Mineral and Bone Metabolism"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.063032"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-3949"},{"key":"e_1_2_7_7_2","first-page":"345","article-title":"Receptor activator of nuclear factor-\u03baB ligand and osteoprotegerin: maintaining the balance to prevent bone loss.","volume":"5","author":"Trouvin A. 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