{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T14:28:31Z","timestamp":1769264911636,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Dried rhizome of<jats:italic>Bergenia ligulata<\/jats:italic>(pashanbhed) is commonly used as a traditional herbal medicine with a wide range of therapeutic applications including urolithiasis. Aqueous extract of<jats:italic>B. ligulata<\/jats:italic>was prepared through maceration followed by decoction (mother extract, 35.9%\u2009w\/w). Further, polarity based fractions were prepared successively from mother extract which yielded 3.4, 2.9, 5.4, 7.5, and 11.3%\u2009w\/w of hexane, toluene, dichloromethane (DCM),<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>n<\/mml:mi><\/mml:mrow><\/mml:math>-butanol, and water fractions, respectively. The in vitro, ex vivo, and real-time antiurolithiasis activity of mother extract and fractions were carried out using aggregation assay in synthetic urine and in rat plasma. The study revealed that DCM fraction has significantly (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>) greater inhibitory potential than other fractions. Ethylene glycol in drinking water (0.75%, v\/v) for 28 days was used for induction of urolithiasis and the curative effects of mother extract and DCM fraction were checked for the level of oxalate, calcium, creatinine, uric acid, and urea of both urine and serum. Treatment with mother extract and DCM fraction at a dose of 185\u2009mg\/kg and 7\u2009mg\/kg, respectively, in ethylene glycol induced rats resulted in a significant (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>) decrease in serum and urine markers. Histological study revealed lower number of calcium oxalate deposits with minimum damage in the kidneys of mother extract and DCM fraction treated rats. This result provides a scientific basis for its traditional claims.<\/jats:p>","DOI":"10.1155\/2017\/1969525","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T16:01:53Z","timestamp":1488470513000},"page":"1-11","source":"Crossref","is-referenced-by-count":23,"title":["Antiurolithiasis Activity of Bioactivity Guided Fraction of<i>Bergenia ligulata<\/i>against Ethylene Glycol Induced Renal Calculi in Rat"],"prefix":"10.1155","volume":"2017","author":[{"given":"Ikshit","family":"Sharma","sequence":"first","affiliation":[{"name":"Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Washim","family":"Khan","sequence":"additional","affiliation":[{"name":"Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabea","family":"Parveen","sequence":"additional","affiliation":[{"name":"Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Javed","family":"Alam","sequence":"additional","affiliation":[{"name":"Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iftekhar","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd Hafizur Rehman","family":"Ansari","sequence":"additional","affiliation":[{"name":"Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, 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