{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T05:35:22Z","timestamp":1768714522556,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["16-15-00091"],"award-info":[{"award-number":["16-15-00091"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>The facultative intracellular pathogen<jats:italic>Listeria monocytogenes<\/jats:italic>causes a severe food-borne infection in humans and animals.<jats:italic>L. monocytogenes<\/jats:italic>invasion factor InlB interacts with the tyrosine kinase c-Met via the N-terminal internalin domain. Previously, distinct variants of the InlB internalin domain (idInlB) have been described in<jats:italic>L. monocytogenes<\/jats:italic>field isolates. Three variants were used to restore full-length InlB expression in the<jats:italic>L. monocytogenes<\/jats:italic>strain EGDe\u0394<jats:italic>inlB<\/jats:italic>. Obtained isogenic<jats:italic>L. monocytogenes<\/jats:italic>strains were tested in the invasion assay and intravenous, intraperitoneal, and intragastric models of infection in mice. All idInlBs were functional, restored InlB activity as an invasion factor, and improved invasion of the parental strain EGDe\u0394inlB into human kidney HEK23 cells. Meanwhile, distinct idInlBs provided different mortality rates and bacterial loads in internal organs. When recombinant strains were compared, the variant designated idInlB14 decreased severity of disease caused by intravenous and intraperitoneal bacterial administration, whereas this variant improved intestine colonization and stimulated intragastric infection. Obtained results demonstrated that naturally occurring idInlBs differed in their impact on severity of<jats:italic>L. monocytogenes<\/jats:italic>infection in mice in dependence on the infection route.<\/jats:p>","DOI":"10.1155\/2017\/2101575","type":"journal-article","created":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T23:33:36Z","timestamp":1514504016000},"page":"1-8","source":"Crossref","is-referenced-by-count":7,"title":["Route of Injection Affects the Impact of InlB Internalin Domain Variants on Severity of<i>Listeria monocytogenes<\/i>Infection in Mice"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7335-5468","authenticated-orcid":true,"given":"Konstantin A.","family":"Sobyanin","sequence":"first","affiliation":[{"name":"Gamaleya Research Center of Epidemiology and Microbiology, Moscow 123098, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena 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