{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:06:02Z","timestamp":1788851162596,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,12]],"date-time":"2017-11-12T00:00:00Z","timestamp":1510444800000},"content-version":"vor","delay-in-days":315,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472134"],"award-info":[{"award-number":["81472134"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>\n                    <jats:italic>Purpose<\/jats:italic>\n                    . To explore the effect and possible mechanism of icariin, a prenylated flavonol glycoside derived from the Chinese herb\n                    <jats:italic>Epimedium sagittatum<\/jats:italic>\n                    that was applied to IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    pretreated human nucleus pulposus (NP) cells.\n                    <jats:italic>Methods<\/jats:italic>\n                    . Human NP cells were isolated from intervertebral discs of patients with scoliosis and lumbar spondylolisthesis. The cells were divided into five groups: A (blank control); B (20\u2009ng\/ml IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    ); C (20\u2009ng\/ml IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    + 20\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M icariin); D (20\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M icariin + 20\u2009ng\/ml IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    + 25\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M LY294002); E (20\u2009ng\/ml IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    + 25\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M LY294002). For each of the five groups, the CCK8, apoptosis rates, ROS rates, and JC\u20101 rates were determined and an electron micrograph was performed. Different expression levels of apoptosis proteins and proteins in the PI3K\/AKT pathway were detected via western blot.\n                    <jats:italic>Results<\/jats:italic>\n                    . We found that the damage effects on human nucleus pulposus cells from 20\u2009ng\/ml of IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    exposure were attenuated by icariin. When the PI3K\/AKT pathway was blocked by LY294002, a specific inhibitor of this pathway, the protective effect of icariin was impaired. In summary, icariin might be a protective traditional Chinese medicine, which prevents inflammation\u2010induced degeneration of intervertebral discs partly through the PI3K\/AKT pathway.\n                  <\/jats:p>","DOI":"10.1155\/2017\/2198323","type":"journal-article","created":{"date-parts":[[2017,11,12]],"date-time":"2017-11-12T18:30:55Z","timestamp":1510511455000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Icariin Prevents IL\u20101\n                    <i>\u03b2<\/i>\n                    \u2010Induced Apoptosis in Human Nucleus Pulposus via the PI3K\/AKT 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